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PMID: 2265610 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M.

The EMBO journal ·Vol. 9 ·No. 13 ·1990-12-00 ·Pages 4347-58

Gerring SL, Spencer F, Hieter P

Abstract

We have analyzed the CTF1 gene, identified in a screen for mutants with decreased chromosome transmission fidelity and shown to correspond to the previously identified chl1 mutation. Chl1 null mutants exhibited a 200-fold increase in the rate of chromosome III missegregation per cell division, and near wild-type rates of marker homozygosis on this chromosome by mitotic recombination. Analysis of the segregation of a marker chromosome indicated that sister chromatid loss (1:0 segregation) and sister chromatid non-disjunction (2:0 segregation) contributed equally to chromosome missegregation. A genomic clone of CHL1 was isolated and used to map its physical position on chromosome XVI. Nucleotide sequence analysis of CHL1 revealed a 2.6 kb open reading frame with a 99 kd predicted protein sequence that contained two PEST sequences and was 23% identical to the coding region of a nucleotide excision repair gene, RAD3. Domains of homology between these two predicted protein sequences included a helix-turn-helix motif and an ATP binding site containing a helicase consensus. Mutants lacking the CHL1 gene product are viable and display two striking, and perhaps interrelated, phenotypes: extreme chromosome instability and a delay in cell cycle progression in G2/M. This delay is independent of the cell cycle checkpoint that requires the function of the RAD9 gene.

Related Genes
CHL1(CTF1) RAD9
MeSH Terms
Alleles Amino Acid Sequence Base Sequence Cell Cycle Chromosomal Proteins, Non-Histone Chromosome Mapping Chromosomes, Fungal/metabolism Cloning, Molecular DNA, Fungal/chemistry Fungal Proteins/genetics Genes, Fungal Genetic Markers Molecular Sequence Data Mutation Phenotype Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Spores, Fungal/analysis
Chemicals
CHL1 protein, S cerevisiae Chromosomal Proteins, Non-Histone DNA, Fungal Fungal Proteins Genetic Markers Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gerring S L
Department of Molecular Biology and Genetics, Johns Hopkins University Medical School, Baltimore, MD 21205.
Spencer F
Hieter P
References (46)
46 references, click to expand
  1. Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis.
    Genetics. 1978 Apr;88(4 Pt 1):651-71 PMID: 17176533
  2. Yeast chromosome replication and segregation.
    Microbiol Rev. 1988 Dec;52(4):568-601 PMID: 3070325
  3. Birth of the D-E-A-D box.
    Nature. 1989 Jan 12;337(6203):121-2 PMID: 2563148
  4. Purification and characterization of Rad3 ATPase/DNA helicase from Saccharomyces cerevisiae.
    J Biol Chem. 1989 Dec 5;264(34):20532-9 PMID: 2555362
  5. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
    J Cell Biol. 1984 Mar;98(3):922-33 PMID: 6365930
  6. RAD3 protein of Saccharomyces cerevisiae is a DNA helicase.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8951-5 PMID: 2827162
  7. Physical mapping of large DNA by chromosome fragmentation.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):6027-31 PMID: 3045811
  8. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  9. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.
    Nucleic Acids Res. 1984 Jul 25;12(14):5647-64 PMID: 6379602
  10. Toxic effects of excess cloned centromeres.
    Mol Cell Biol. 1986 Jun;6(6):2213-22 PMID: 3537715
  11. Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage.
    Mol Cell Biol. 1989 May;9(5):1882-96 PMID: 2664461
  12. A genetic analysis of dicentric minichromosomes in Saccharomyces cerevisiae.
    Cell. 1987 Mar 13;48(5):801-12 PMID: 3545498
  13. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  14. Flow cytometric determinations of cellular substances in algae, bacteria, moulds and yeasts.
    Antonie Van Leeuwenhoek. 1978;44(3-4):269-82 PMID: 378116
  15. Analysis of the essential and excision repair functions of the RAD3 gene of Saccharomyces cerevisiae by mutagenesis.
    Mol Cell Biol. 1986 Apr;6(4):1218-27 PMID: 3023877
  16. The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae.
    Science. 1988 Jul 15;241(4863):317-22 PMID: 3291120
  17. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  18. Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss.
    Cell. 1985 Feb;40(2):381-92 PMID: 3967296
  19. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  20. Conservation of mitotic controls in fission and budding yeasts.
    Cell. 1989 Apr 21;57(2):295-303 PMID: 2649252
  21. The RAD3 gene of Saccharomyces cerevisiae encodes a DNA-dependent ATPase.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6045-9 PMID: 2957691
  22. Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae.
    Genetics. 1990 Feb;124(2):237-49 PMID: 2407610
  23. Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis.
    Science. 1986 Oct 17;234(4774):364-8 PMID: 2876518
  24. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  25. Bisexual mating behavior in a diploid of Saccharomyces cerevisiae: evidence for genetically controlled non-random chromosome loss during vegetative growth.
    Genetics. 1974 Nov;78(3):843-58 PMID: 4615978
  26. Checkpoints: controls that ensure the order of cell cycle events.
    Science. 1989 Nov 3;246(4930):629-34 PMID: 2683079
  27. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  28. Altered fidelity of mitotic chromosome transmission in cell cycle mutants of S. cerevisiae.
    Genetics. 1985 Jul;110(3):381-95 PMID: 3894160
  29. RAD3 gene of Saccharomyces cerevisiae: nucleotide sequence of wild-type and mutant alleles, transcript mapping, and aspects of gene regulation.
    Mol Cell Biol. 1985 Jan;5(1):17-26 PMID: 3885009
  30. Mutations of Bacteria from Virus Sensitivity to Virus Resistance.
    Genetics. 1943 Nov;28(6):491-511 PMID: 17247100
  31. Mutation of lysine-48 to arginine in the yeast RAD3 protein abolishes its ATPase and DNA helicase activities but not the ability to bind ATP.
    EMBO J. 1988 Oct;7(10):3263-9 PMID: 2846277
  32. Positional mapping of genes by chromosome blotting and chromosome fragmentation.
    Methods Enzymol. 1991;194:57-77 PMID: 2005810
  33. The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog.
    EMBO J. 1988 Dec 20;7(13):4335-46 PMID: 2907481
  34. Visual assay for chromosome ploidy.
    Methods Enzymol. 1987;155:351-72 PMID: 3431466
  35. Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae.
    Microbiol Rev. 1988 Mar;52(1):70-102 PMID: 3280967
  36. Rapid DNA isolations for enzymatic and hybridization analysis.
    Methods Enzymol. 1980;65(1):404-11 PMID: 6246361
  37. DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Nov;8(11):4675-84 PMID: 3062366
  38. Establishing homologies in protein sequences.
    Methods Enzymol. 1983;91:524-45 PMID: 6855599
  39. A chicken-yeast chimeric beta-tubulin protein is incorporated into mouse microtubules in vivo.
    Cell. 1986 Feb 14;44(3):461-8 PMID: 3753663
  40. Unidirectional digestion with exonuclease III in DNA sequence analysis.
    Methods Enzymol. 1987;155:156-65 PMID: 3323819
  41. The nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae: a potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region.
    Nucleic Acids Res. 1985 Apr 11;13(7):2357-72 PMID: 2987851
  42. The selection of S. cerevisiae mutants defective in the start event of cell division.
    Genetics. 1980 Jul;95(3):561-77 PMID: 7002718
  43. Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):735-9 PMID: 3003748
  44. Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA.
    EMBO J. 1987 May;6(5):1441-9 PMID: 3038524
  45. Insertions of up to 17 amino acids into a region of alpha-tubulin do not disrupt function in vivo.
    Mol Cell Biol. 1987 Oct;7(10):3799-805 PMID: 3316988
  46. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-12-00
Pages
4347-58
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552222
Subset
IM
Grants
NCI NIH HHS · 5T32CA09139-15 · United States
NCI NIH HHS · CA16519 · United States
External Links
PubMed source PMC Full Text View journal details
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