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PMID: 9472022 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Vaccinia NPH-I, a DExH-box ATPase, is the energy coupling factor for mRNA transcription termination.

Genes & development ·Vol. 12 ·No. 4 ·1998-02-15 ·Pages 538-46

Deng L, Shuman S

Abstract

Vaccinia virus RNA polymerase terminates transcription in response to a specific signal UUUUUNU in the nascent RNA. Transduction of this signal to the elongating polymerase requires a trans-acting viral termination factor (VTF/capping enzyme), and is coupled to the hydrolysis of ATP. Recent studies suggest that ATP hydrolysis is catalyzed by a novel termination protein (factor X), which is tightly associated with the elongation complex. Here, we identify factor X as NPH-I (nucleoside triphosphate phosphohydrolase-I), a virus-encoded DNA-dependent ATPase of the DExH-box family. We report that NPH-I serves two roles in transcription (1) it acts in concert with VTF/CE to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex, and (2) it acts by itself as a polymerase elongation factor to facilitate readthrough of intrinsic pause sites. A mutation (K61A) in the GxGKT motif of NPH-I abolishes ATP hydrolysis and eliminates the termination and elongation factor activities. Related DExH proteins may have similar roles at postinitiation steps during cellular mRNA synthesis.

MeSH Terms
Acid Anhydride Hydrolases/classification,genetics,metabolism Adenosine Triphosphatases/classification,genetics,metabolism Amino Acid Sequence Energy Metabolism Heparin/pharmacology Models, Genetic Molecular Sequence Data Nucleoside-Triphosphatase RNA, Messenger/metabolism Recombinant Proteins/metabolism Terminator Regions, Genetic Transcription, Genetic/drug effects Vaccinia virus/enzymology,genetics Viral Proteins/genetics,metabolism
Chemicals
RNA, Messenger Recombinant Proteins Viral Proteins Heparin Acid Anhydride Hydrolases Adenosine Triphosphatases Nucleoside-Triphosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deng L
Molecular Biology Program, Sloan-Kettering Institute, New York, New York 10021, USA.
Shuman S
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.4.538
Published
1998-02-15
Pages
538-46
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316528
Subset
IM
Grants
NIGMS NIH HHS · GM42498 · United States
External Links
PubMed source DOI: 10.1101/gad.12.4.538 PMC Full Text View journal details
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