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PMID: 10816431 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of Bacillus subtilis CsaA with SecA and precursor proteins.

The Biochemical journal ·Vol. 348 Pt 2 ·2000-06-01 ·Pages 367-73

Müller JP, Ozegowski J, Vettermann S, Swaving J, Van Wely KH, Driessen AJ

Abstract

CsaA from the Gram-positive bacterium Bacillus subtilis has been identified previously as a suppressor of the growth and protein-export defect of Escherichia coli secA(Ts) mutants. CsaA has chaperone-like activities in vivo and in vitro. To examine the role of CsaA in protein export in B. subtilis, expression of the csaA gene was repressed. While export of most proteins remained unaffected, export of at least two proteins was significantly reduced upon CsaA depletion. CsaA co-immunoprecipitates and co-purifies with the SecA proteins of E. coli and B. subtilis, and binds the B. subtilis preprotein prePhoB. Purified CsaA stimulates the translocation of prePhoB into E. coli membrane vesicles bearing the B. subtilis translocase, whereas it interferes with the SecB-mediated translocation of proOmpA into membrane vesicles of E. coli. The specific interaction with the SecA translocation ATPase and preproteins suggests that CsaA acts as a chaperone that promotes the export of a subset of preproteins in B. subtilis.

MeSH Terms
Adenosine Triphosphatases/isolation & purification,metabolism Bacillus subtilis/genetics,metabolism Bacterial Proteins/genetics,isolation & purification,metabolism Carrier Proteins/isolation & purification,metabolism DNA-Binding Proteins/isolation & purification,metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Genotype Membrane Transport Proteins Molecular Chaperones Phenotype Plasmids Protein Binding Protein Precursors/isolation & purification,metabolism Recombinant Proteins/isolation & purification,metabolism SEC Translocation Channels SecA Proteins
Chemicals
Bacterial Proteins Carrier Proteins DNA-Binding Proteins Escherichia coli Proteins Membrane Transport Proteins Molecular Chaperones Protein Precursors Recombinant Proteins SEC Translocation Channels PhoB protein, Bacteria CsaA protein, Bacillus subtilis Adenosine Triphosphatases SecA Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Müller J P
Institute for Molecular Biology, Jena University, Winzerlaer Strasse 10, D-07745 Jena, Germany. jmueller@imb-jena.de
Ozegowski J
Vettermann S
Swaving J
Van Wely K H
Driessen A J
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Email
jmueller@imb-jena.de
Published
2000-06-01
Pages
367-73
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1221075
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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