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PMID: 1671351 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Heat-shock proteins can substitute for SecB function during protein export in Escherichia coli.

The EMBO journal ·Vol. 10 ·No. 2 ·1991-02-00 ·Pages 239-45

Altman E, Kumamoto CA, Emr SD

Abstract

In this study we have shown that (i) induction of the heat-shock response can substitute for SecB function in Escherichia coli, (ii) SecB itself is not a heat-shock protein and (iii) a basal level of heat-shock proteins is required for cells to grow in the absence of a functional SecB protein. Overproduction of DnaK, or GroEL/ES, which were candidates for the heat-shock proteins that could substitute for SecB function, did not rescue the export defect caused when SecB was limiting or absent. In an attempt to identify the heat-shock protein(s) which could substitute for SecB function, unlinked suppressors of secB were isolated and characterized. Interestingly, most of the suppressors mapped to the rpoH locus. Since rpoH encodes sigma 32, the heat-shock transcription factor, it is likely that these suppressors affect the synthesis levels of heat-shock proteins that can substitute for SecB function. The remaining suppressors did not map to any known heat-shock or export genes. Collectively, our data suggest that these suppressors may represent unidentified heat-shock proteins or export factors that act in a manner similar to SecB in facilitating the export process in E. coli.

MeSH Terms
Bacterial Proteins/genetics,metabolism Chaperonin 60 Escherichia coli/genetics,metabolism Escherichia coli Proteins Genetic Linkage Genotype HSP70 Heat-Shock Proteins Heat-Shock Proteins/genetics,metabolism Protein Processing, Post-Translational Recombination, Genetic Suppression, Genetic
Chemicals
Bacterial Proteins Chaperonin 60 Escherichia coli Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins SecB protein, Bacteria dnaK protein, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Altman E
Division of Biology, California Institute of Technology, Pasadena 91125.
Kumamoto C A
Emr S D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-02-00
Pages
239-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452639
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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