Home LiteratureArticle Details
PMID: 10747090 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of the COOH terminus of Sec2p in the transport of post-Golgi vesicles.

The Journal of cell biology ·Vol. 149 ·No. 1 ·2000-04-03 ·Pages 95-110

Elkind NB, Walch-Solimena C, Novick PJ

Abstract

Sec2p is required for the polarized transport of secretory vesicles in S. cerevisiae. The Sec2p NH(2) terminus encodes an exchange factor for the Rab protein Sec4p. Sec2p associates with vesicles and in Sec2p COOH-terminal mutants Sec4p and vesicles no longer accumulate at bud tips. Thus, the Sec2p COOH terminus functions in targeting vesicles, however, the mechanism of function is unknown. We found comparable exchange activity for truncated and full-length Sec2 proteins, implying that the COOH terminus does not alter the exchange rate. Full-length Sec2-GFP, similar to Sec4p, concentrates at bud tips. A COOH-terminal 58-amino acid domain is necessary but not sufficient for localization. Sec2p localization depends on actin, Myo2p and Sec1p, Sec6p, and Sec9p function. Full-length, but not COOH-terminally truncated Sec2 proteins are enriched on membranes. Membrane association of full-length Sec2p is reduced in sec6-4 and sec9-4 backgrounds at 37 degrees C but unaffected at 25 degrees C. Taken together, these data correlate loss of localization of Sec2 proteins with reduced membrane association. In addition, Sec2p membrane attachment is substantially Sec4p independent, supporting the notion that Sec2p interacts with membranes via an unidentified Sec2p receptor, which would increase the accessibility of Sec2p exchange activity for Sec4p.

MeSH Terms
Actins/metabolism Biological Transport Cell Division Cell Membrane/chemistry,metabolism Cell Polarity Cytoplasmic Granules/metabolism Cytoskeleton/metabolism Fungal Proteins/genetics,metabolism GTP-Binding Proteins/chemistry,genetics,metabolism Genes, Fungal/genetics,physiology Golgi Apparatus/metabolism Guanine Nucleotide Exchange Factors Kinetics Phosphorylation Protein Binding Recombinant Fusion Proteins/chemistry,genetics,metabolism Saccharomyces cerevisiae/cytology,enzymology,genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Deletion/genetics Temperature Vacuoles/metabolism rab GTP-Binding Proteins/genetics,metabolism
Chemicals
Actins Fungal Proteins Guanine Nucleotide Exchange Factors Recombinant Fusion Proteins SEC2 protein, S cerevisiae Saccharomyces cerevisiae Proteins GTP-Binding Proteins SEC4 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elkind N B
Department of Cell Biology, Yale University, New Haven, Connecticut, 06510, USA.
Walch-Solimena C
Novick P J
References (51)
51 references, click to expand
  1. Motivating endosome motility.
    Nat Cell Biol. 1999 Oct;1(6):E145-7 PMID: 10559973
  2. Interaction of Sec4 with GDI proteins from bovine brain, Drosophila melanogaster and Saccharomyces cerevisiae. Conservation of GDI membrane dissociation activity.
    FEBS Lett. 1993 Oct 4;331(3):233-8 PMID: 8375508
  3. Role of actin and Myo2p in polarized secretion and growth of Saccharomyces cerevisiae.
    Mol Biol Cell. 2000 May;11(5):1727-37 PMID: 10793147
  4. A syndrome associating partial albinism and immunodeficiency.
    Am J Med. 1978 Oct;65(4):691-702 PMID: 707528
  5. Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1858-62 PMID: 377286
  6. Friends and family: the role of the Rab GTPases in vesicular traffic.
    Cell. 1993 Nov 19;75(4):597-601 PMID: 8242735
  7. GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway.
    EMBO J. 1994 Apr 1;13(7):1718-28 PMID: 8157010
  8. Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae.
    J Cell Biol. 1994 May;125(4):825-42 PMID: 8188749
  9. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis.
    Cell. 1994 Oct 21;79(2):245-58 PMID: 7954793
  10. Partial albinism with immunodeficiency (Griscelli syndrome).
    J Pediatr. 1994 Dec;125(6 Pt 1):886-95 PMID: 7996360
  11. The role of Myo2, a yeast class V myosin, in vesicular transport.
    J Cell Biol. 1995 Mar;128(6):1055-68 PMID: 7896871
  12. Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae.
    J Cell Biol. 1995 Jul;130(2):299-312 PMID: 7615633
  13. Expression, purification, and assays of Gdi1p from recombinant Escherichia coli.
    Methods Enzymol. 1995;257:232-40 PMID: 8583926
  14. Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion.
    Cell. 1995 Nov 3;83(3):423-32 PMID: 8521472
  15. FACS-optimized mutants of the green fluorescent protein (GFP).
    Gene. 1996;173(1 Spec No):33-8 PMID: 8707053
  16. GTPase activity of Rab5 acts as a timer for endocytic membrane fusion.
    Nature. 1996 Sep 19;383(6597):266-9 PMID: 8805704
  17. Cultured melanocytes from dilute mutant mice exhibit dendritic morphology and altered melanosome distribution.
    Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14554-8 PMID: 8962090
  18. The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae.
    EMBO J. 1996 Dec 2;15(23):6483-94 PMID: 8978675
  19. Characterization of the yeast transcriptome.
    Cell. 1997 Jan 24;88(2):243-51 PMID: 9008165
  20. High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A.
    J Cell Biol. 1997 Apr 21;137(2):399-416 PMID: 9128251
  21. Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles.
    J Cell Biol. 1997 Jun 30;137(7):1495-509 PMID: 9199166
  22. Griscelli disease maps to chromosome 15q21 and is associated with mutations in the myosin-Va gene.
    Nat Genet. 1997 Jul;16(3):289-92 PMID: 9207796
  23. Identification of genes controlling growth polarity in the budding yeast Saccharomyces cerevisiae: a possible role of N-glycosylation and involvement of the exocyst complex.
    Genetics. 1997 Oct;147(2):421-34 PMID: 9335583
  24. The predominant defect in dilute melanocytes is in melanosome distribution and not cell shape, supporting a role for myosin V in melanosome transport.
    J Muscle Res Cell Motil. 1997 Oct;18(5):517-27 PMID: 9350005
  25. Interaction of a Golgi-associated kinesin-like protein with Rab6.
    Science. 1998 Jan 23;279(5350):580-5 PMID: 9438855
  26. Smy1p, a kinesin-related protein that does not require microtubules.
    J Cell Biol. 1998 Feb 23;140(4):873-83 PMID: 9472039
  27. Sec3p is a spatial landmark for polarized secretion in budding yeast.
    Cell. 1998 Feb 20;92(4):559-71 PMID: 9491896
  28. Two distinct effectors of the small GTPase Rab5 cooperate in endocytic membrane fusion.
    EMBO J. 1998 Apr 1;17(7):1930-40 PMID: 9524116
  29. The terminal tail region of a yeast myosin-V mediates its attachment to vacuole membranes and sites of polarized growth.
    Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14799-804 PMID: 9843969
  30. Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function In vivo.
    J Cell Biol. 1998 Dec 28;143(7):1899-918 PMID: 9864363
  31. Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast.
    J Cell Biol. 1998 Dec 28;143(7):1931-45 PMID: 9864365
  32. Molecular motors join forces.
    Nature. 1999 Jan 21;397(6716):204-5 PMID: 9930690
  33. The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis.
    EMBO J. 1999 Feb 15;18(4):1071-80 PMID: 10022848
  34. The tail of a yeast class V myosin, myo2p, functions as a localization domain.
    Mol Biol Cell. 1999 Apr;10(4):1001-17 PMID: 10198053
  35. Sec1p binds to SNARE complexes and concentrates at sites of secretion.
    J Cell Biol. 1999 Jul 26;146(2):333-44 PMID: 10427089
  36. Exo84p is an exocyst protein essential for secretion.
    J Biol Chem. 1999 Aug 13;274(33):23558-64 PMID: 10438536
  37. Differential effect of cytochalasin B on the aggregation of melanosomes in cultured mouse melanoma cells.
    Anat Rec. 1980;196(4):449-59 PMID: 7396234
  38. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.
    Cell. 1980 Aug;21(1):205-15 PMID: 6996832
  39. Order of events in the yeast secretory pathway.
    Cell. 1981 Aug;25(2):461-9 PMID: 7026045
  40. Latrunculins: novel marine toxins that disrupt microfilament organization in cultured cells.
    Science. 1983 Feb 4;219(4584):493-5 PMID: 6681676
  41. Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.
    J Cell Biol. 1984 Mar;98(3):934-45 PMID: 6365931
  42. Phenotypic analysis of temperature-sensitive yeast actin mutants.
    Cell. 1985 Feb;40(2):405-16 PMID: 3967297
  43. Inhibition of actin polymerization by latrunculin A.
    FEBS Lett. 1987 Mar 23;213(2):316-8 PMID: 3556584
  44. A ras-like protein is required for a post-Golgi event in yeast secretion.
    Cell. 1987 May 22;49(4):527-38 PMID: 3552249
  45. Purification and characterization of constitutive secretory vesicles from yeast.
    J Cell Biol. 1987 Jul;105(1):163-74 PMID: 3301865
  46. Sec2 protein contains a coiled-coil domain essential for vesicular transport and a dispensable carboxy terminal domain.
    J Cell Biol. 1990 Jun;110(6):1897-909 PMID: 1693620
  47. The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles.
    J Cell Biol. 1991 May;113(3):539-51 PMID: 2016335
  48. Griscelli disease with cerebral involvement.
    Eur J Pediatr. 1991 Apr;150(6):419-22 PMID: 1828234
  49. Suppression of a myosin defect by a kinesin-related gene.
    Nature. 1992 Mar 26;356(6367):358-61 PMID: 1549181
  50. A kindred with Griscelli disease: spectrum of neurological involvement.
    Eur J Pediatr. 1993 May;152(5):402-5 PMID: 8319705
  51. The COOH-terminal domain of Myo2p, a yeast myosin V, has a direct role in secretory vesicle targeting.
    J Cell Biol. 1999 Nov 15;147(4):791-808 PMID: 10562281
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.149.1.95
Published
2000-04-03
Pages
95-110
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2175086
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.149.1.95 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2