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PMID: 10702402 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel association between the human heat shock transcription factor 1 (HSF1) and prostate adenocarcinoma.

The American journal of pathology ·Vol. 156 ·No. 3 ·2000-03-00 ·Pages 857-64

Hoang AT, Huang J, Rudra-Ganguly N, Zheng J, Powell WC, Rabindran SK, Wu C, Roy-Burman P

Abstract

A search for differentially expressed genes in a pair of nonmetastatic (PC-3) versus metastatic variant (PC-3M) human prostate carcinoma cell lines led to identification of the human heat shock factor (HSF1) as an overexpressed gene product in PC-3M cells. Analysis of primary prostate cancer specimens indicated that HSF1 is generally up-regulated in most of the malignant prostate epithelial cells relative to the normal prostate cells. Among the known effectors of HSF1 action, constitutive levels of HSP70 and HSP90 are not significantly altered by the naturally elevated expression of HSF1 as in PC-3M cells or by transduced overexpression of HSF1 in PC-3 cells. The basal levels of HSP27 in both cases are, however, consistently increased by two- to threefold. With respect to response to heat shock, high basal concentration of HSP90 is not further enhanced in these cells, and HSP70 is up-regulated irrespective of HSF1 level. Heat shock, however, causes an increase in HSP27 when HSF1 is up-regulated, except when the expression of HSF1 is already too high. These results document for the first time that HSF1 is overexpressed in human prostate cancer cells, at least one consequence of which in the prostate cancer cell lines tested is stimulation of both basal and stress-induced expression of HSP27, an important factor in cell growth, differentiation, or apoptosis.

MeSH Terms
Adenocarcinoma/genetics,metabolism,secondary Blotting, Northern Blotting, Western Cloning, Molecular DNA-Binding Proteins/genetics,metabolism HSP27 Heat-Shock Proteins HSP70 Heat-Shock Proteins/metabolism HSP90 Heat-Shock Proteins/metabolism Heat Shock Transcription Factors Heat-Shock Proteins/genetics,metabolism Hot Temperature Humans Male Molecular Chaperones Neoplasm Proteins/metabolism Prostate/metabolism Prostatic Neoplasms/genetics,metabolism,pathology RNA, Messenger/metabolism RNA, Neoplasm/analysis Transcription Factors Tumor Cells, Cultured/metabolism Up-Regulation
Chemicals
DNA-Binding Proteins HSF1 protein, human HSP27 Heat-Shock Proteins HSP70 Heat-Shock Proteins HSP90 Heat-Shock Proteins HSPB1 protein, human Heat Shock Transcription Factors Heat-Shock Proteins Molecular Chaperones Neoplasm Proteins RNA, Messenger RNA, Neoplasm Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Hoang A T
Department of Pathology, University of Southern California School of Medicine, Los Angeles, California 90033, USA.
Huang J
Rudra-Ganguly N
Zheng J
Powell W C
Rabindran S K
Wu C
Roy-Burman P
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)64954-1
Published
2000-03-00
Pages
857-64
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1876857
Subset
IM
Grants
NCI NIH HHS · R01 CA059705 · United States
NCI NIH HHS · CA59705 · United States
NIAID NIH HHS · T32-AI07078 · United States
NIAID NIH HHS · T32 AI007078 · United States
NCI NIH HHS · T32 CA009320 · United States
NCI NIH HHS · T32-CA09320 · United States
External Links
PubMed source DOI: 10.1016/S0002-9440(10)64954-1 PMC Full Text View journal details
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