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PMID: 2071672 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 25-kD inhibitor of actin polymerization is a low molecular mass heat shock protein.

The Journal of cell biology ·Vol. 114 ·No. 2 ·1991-07-00 ·Pages 255-61

Miron T, Vancompernolle K, Vandekerckhove J, Wilchek M, Geiger B

Abstract

The 25-kD inhibitor of actin polymerization (25-kD IAP), isolated from turkey smooth muscle (Miron, T., M. Wilchek, and B. Geiger, 1988. Eur. J. Biochem. 178:543-553), is shown here to be a low molecular mass heat shock protein (HSP). Direct sequence analysis of the purified protein, as well as cloning and sequencing of the respective cDNA, disclosed a high degree of homology (67% identity, 80% similarity) to the human 27-kD HSP. Southern blot of chicken genomic DNA disclosed one band, suggesting the presence of a single gene, and Northern blot analysis revealed abundant transcript of approximately 1 kb in gizzard and heart tissues and lower amounts in total 18-d chick embryo RNA and in cultured fibroblasts. Exposure of the latter cells to 45 degrees C resulted in over 15-fold increase in the apparent level of the 25-kD IAP protein, confirming that its expression is regulated by heat shock. Immunofluorescent microscopic localization indicated that after heat treatment, the levels of the 25-kD IAP were markedly increased and the protein was apparently associated with cytoplasmic granules. Heat shock also had a transient, yet prominent, effect on the microfilament system in cultured fibroblasts: stress fibers disintegrated within 10-15 min after incubation at 45 degrees C, yet upon further incubation at the elevated temperature, conspicuous actin bundles were apparently reformed.

MeSH Terms
Actin Cytoskeleton/drug effects,ultrastructure Actins/antagonists & inhibitors,metabolism Amino Acid Sequence Animals Avian Proteins Base Sequence Blotting, Northern Blotting, Southern Cells, Cultured Chick Embryo Cytoplasmic Granules/metabolism,ultrastructure Fibroblasts/metabolism,ultrastructure Fluorescent Antibody Technique Heat-Shock Proteins/genetics,isolation & purification,metabolism,pharmacology Molecular Sequence Data Molecular Weight Muscle, Smooth/chemistry,metabolism Polymers Temperature Time Factors Turkeys
Chemicals
Actins Avian Proteins Heat-Shock Proteins Polymers actin polymerization inhibitor 25 kDa protein, Meleagris gallopavo
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Miron T
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Vancompernolle K
Vandekerckhove J
Wilchek M
Geiger B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.114.2.255
Published
1991-07-00
Pages
255-61
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289078
Subset
IM
Databases
GENBANK
M60691, M60692, M60693, M60694, M60695, M60696, M60697, S55286, X56699, X59541
External Links
PubMed source DOI: 10.1083/jcb.114.2.255 PMC Full Text View journal details
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