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PMID: 10698943 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hormone activation induces nucleosome positioning in vivo.

The EMBO journal ·Vol. 19 ·No. 5 ·2000-03-01 ·Pages 1023-33

Belikov S, Gelius B, Almouzni G, Wrange O

Abstract

The mouse mammary tumor virus (MMTV) promoter is induced by glucocorticoid hormone. A robust hormone- and receptor-dependent activation could be reproduced in Xenopus laevis oocytes. The homogeneous response in this system allowed a detailed analysis of the transition in chromatin structure following hormone activation. This revealed two novel findings: hormone activation led to the establishment of specific translational positioning of nucleosomes despite the lack of significant positioning in the inactive state; and, in the active promoter, a subnucleosomal particle encompassing the glucocorticoid receptor (GR)-binding region was detected. The presence of only a single GR-binding site was sufficient for the structural transition to occur. Both basal promoter elements and ongoing transcription were dispensable. These data reveal a stepwise process in the transcriptional activation by glucocorticoid hormone.

MeSH Terms
Animals Female Gene Expression Regulation Glucocorticoids/genetics,metabolism Mammary Tumor Virus, Mouse/genetics Mice Nucleosomes/genetics,metabolism Oocytes Promoter Regions, Genetic Xenopus laevis
Chemicals
Glucocorticoids Nucleosomes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Belikov S
Laboratory of Molecular Genetics, Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, SE-17177 Stockholm, Sweden.
Gelius B
Almouzni G
Wrange O
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.5.1023
Published
2000-03-01
Pages
1023-33
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305641
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.5.1023 PMC Full Text View journal details
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