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PMID: 10655215 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae.

Genetics ·Vol. 154 ·No. 2 ·2000-02-00 ·Pages 609-22

Lorenz MC, Pan X, Harashima T, Cardenas ME, Xue Y, Hirsch JP, Heitman J

Abstract

Pseudohyphal differentiation in the budding yeast Saccharomyces cerevisiae is induced in diploid cells in response to nitrogen starvation and abundant fermentable carbon source. Filamentous growth requires at least two signaling pathways: the pheromone responsive MAP kinase cascade and the Gpa2p-cAMP-PKA signaling pathway. Recent studies have established a physical and functional link between the Galpha protein Gpa2 and the G protein-coupled receptor homolog Gpr1. We report here that the Gpr1 receptor is required for filamentous and haploid invasive growth and regulates expression of the cell surface flocculin Flo11. Epistasis analysis supports a model in which the Gpr1 receptor regulates pseudohyphal growth via the Gpa2p-cAMP-PKA pathway and independently of both the MAP kinase cascade and the PKA related kinase Sch9. Genetic and physiological studies indicate that the Gpr1 receptor is activated by glucose and other structurally related sugars. Because expression of the GPR1 gene is known to be induced by nitrogen starvation, the Gpr1 receptor may serve as a dual sensor of abundant carbon source (sugar ligand) and nitrogen starvation. In summary, our studies reveal a novel G protein-coupled receptor senses nutrients and regulates the dimorphic transition to filamentous growth via a Galpha protein-cAMP-PKA signal transduction cascade.

MeSH Terms
Carbohydrate Metabolism Cell Differentiation Cyclic AMP/biosynthesis Fermentation Fungal Proteins/metabolism Gene Expression Regulation, Fungal Haploidy Membrane Glycoproteins Membrane Proteins/genetics Receptors, Cell Surface/physiology Receptors, G-Protein-Coupled Saccharomyces cerevisiae/cytology,growth & development,metabolism Saccharomyces cerevisiae Proteins Signal Transduction
Chemicals
FLO11 protein, S cerevisiae Fungal Proteins GPR1 protein, S cerevisiae Membrane Glycoproteins Membrane Proteins Receptors, Cell Surface Receptors, G-Protein-Coupled Saccharomyces cerevisiae Proteins Cyclic AMP
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lorenz M C
Departments of Genetics, Pharmacology and Cancer Biology, Microbiology, and Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.
Pan X
Harashima T
Cardenas M E
Xue Y
Hirsch J P
Heitman J
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/154.2.609
Published
2000-02-00
Pages
609-22
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460933
Subset
IM
Grants
NCI NIH HHS · CA77075 · United States
External Links
PubMed source DOI: 10.1093/genetics/154.2.609 PMC Full Text View journal details
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