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PMID: 10233147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 10 ·No. 5 ·1999-05-00 ·Pages 1325-35

Mösch HU, Kübler E, Krappmann S, Fink GR, Braus GH

Abstract

The two highly conserved RAS genes of the budding yeast Saccharomyces cerevisiae are redundant for viability. Here we show that haploid invasive growth development depends on RAS2 but not RAS1. Ras1p is not sufficiently expressed to induce invasive growth. Ras2p activates invasive growth using either of two downstream signaling pathways, the filamentation MAPK (Cdc42p/Ste20p/MAPK) cascade or the cAMP-dependent protein kinase (Cyr1p/cAMP/PKA) pathway. This signal branch point can be uncoupled in cells expressing Ras2p mutant proteins that carry amino acid substitutions in the adenylyl cyclase interaction domain and therefore activate invasive growth solely dependent on the MAPK cascade. Both Ras2p-controlled signaling pathways stimulate expression of the filamentation response element-driven reporter gene depending on the transcription factors Ste12p and Tec1p, indicating a crosstalk between the MAPK and the cAMP signaling pathways in haploid cells during invasive growth.

MeSH Terms
Adenylyl Cyclases/metabolism Amino Acid Sequence Binding Sites Calcium-Calmodulin-Dependent Protein Kinases/metabolism Cell Cycle Proteins/genetics,metabolism Conserved Sequence Cyclic AMP/metabolism Cyclic AMP-Dependent Protein Kinases/metabolism DNA-Binding Proteins/genetics,metabolism FMN Reductase Fungal Proteins/genetics,metabolism GTP-Binding Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Reporter Genes, Suppressor Haploidy Intracellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases Molecular Sequence Data Mutation NADH, NADPH Oxidoreductases/genetics,metabolism Protein Serine-Threonine Kinases/genetics,metabolism Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Transcription Factors/genetics,metabolism cdc42 GTP-Binding Protein, Saccharomyces cerevisiae ras Proteins/genetics,metabolism
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Fungal Proteins Intracellular Signaling Peptides and Proteins STE12 protein, S cerevisiae Saccharomyces cerevisiae Proteins TEC1 protein, S cerevisiae Transcription Factors Cyclic AMP FMN Reductase NADH, NADPH Oxidoreductases ferric citrate iron reductase Protein Serine-Threonine Kinases Cyclic AMP-Dependent Protein Kinases Calcium-Calmodulin-Dependent Protein Kinases MAP Kinase Kinase Kinases STE20 protein, S cerevisiae GTP-Binding Proteins RAS1 protein, S cerevisiae RAS2 protein, S cerevisiae cdc42 GTP-Binding Protein, Saccharomyces cerevisiae ras Proteins Adenylyl Cyclases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mösch H U
Institute for Microbiology and Genetics, Georg-August-University, D-37077 Göttingen, Germany.
Kübler E
Krappmann S
Fink G R
Braus G H
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.10.5.1325
Published
1999-05-00
Pages
1325-35
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25273
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.10.5.1325 PMC Full Text View journal details
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