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PMID: 10648530 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Insertional inactivation of genes encoding components of the sodium-type flagellar motor and switch of Vibrio parahaemolyticus.

Journal of bacteriology ·Vol. 182 ·No. 4 ·2000-02-00 ·Pages 1035-45

Boles BR, McCarter LL

Abstract

Vibrio parahaemolyticus possesses two types of flagella, polar and lateral, powered by distinct energy sources, which are derived from the sodium and proton motive forces, respectively. Although proton-powered flagella in Escherichia coli and Salmonella enterica serovar Typhimurium have been extensively studied, the mechanism of torque generation is still not understood. Molecular knowledge of the structure of the sodium-driven motor is only now being developed. In this work, we identify the switch components, FliG, FliM, and FliN, of the sodium-type motor. This brings the total number of genes identified as pertinent to polar motor function to seven. Both FliM and FliN possess charged domains not found in proton-type homologs; however, they can interact with the proton-type motor of E. coli to a limited extent. Residues known to be critical for torque generation in the proton-type motor are conserved in the sodium-type motor, suggesting a common mechanism for energy transfer at the rotor-stator interface regardless of the driving force powering rotation. Mutants representing a complete panel of insertionally inactivated switch and motor genes were constructed. All of these mutants were defective in sodium-driven swimming motility. Alkaline phosphatase could be fused to the C termini of MotB and MotY without abolishing motility, whereas deletion of the unusual, highly charged C-terminal domain of FliM disrupted motor function. All of the mutants retained proton-driven, lateral motility over surfaces. Thus, although central chemotaxis genes are shared by the polar and lateral systems, genes encoding the switch components, as well as the motor genes, are distinct for each motility system.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism DNA Transposable Elements Flagella/genetics,physiology Genetic Complementation Test Immunoblotting Molecular Motor Proteins/genetics,physiology Molecular Sequence Data Mutagenesis, Insertional Plasmids/genetics Sequence Alignment Sequence Analysis, DNA Sodium/pharmacology Vibrio parahaemolyticus/genetics,physiology
Chemicals
Bacterial Proteins DNA Transposable Elements FliN protein, Bacteria Flig protein, Bacteria Molecular Motor Proteins FliM protein, Bacteria Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boles B R
Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA.
McCarter L L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.4.1035-1045.2000
Published
2000-02-00
Pages
1035-45
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94380
Subset
IM
Grants
NIGMS NIH HHS · GM43196 · United States
Databases
GENBANK
AF069392
External Links
PubMed source DOI: 10.1128/JB.182.4.1035-1045.2000 PMC Full Text View journal details
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