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PMID: 4598295 Published · ppublish English Journal Article

Chemomechanical coupling without ATP: the source of energy for motility and chemotaxis in bacteria.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 4 ·1974-04-00 ·Pages 1239-43

Larsen SH, Adler J, Gargus JJ, Hogg RW

Abstract

The source of energy for bacterial motility is the intermediate in oxidative phosphorylation, not ATP directly. For chemotaxis, however, there is an additional requirement, presumably ATP. These conclusions are based on the following findings. (i) Unlike their parents, mutants of Escherichia coli and Salmonella typhimurium that are blocked in the conversion of ATP to the intermediate of oxidative phosphorylation failed to swim anaerobically, even when they produced ATP. When respiration was restored to the mutants, motility was simultaneously restored. (ii) Carbonylcyanide m-chlorophenylhydrazone, which uncouples oxidative phosphorylation, completely inhibited motility even though ATP remained present. (iii) Arsenate did not inhibit motility in the presence of an oxidizable substrate, though it did reduce ATP levels to less than 0.3% (iv) Arsenate completely inhibited chemotaxis under conditions where motility was normal.

MeSH Terms
Adenosine Triphosphate/metabolism Anaerobiosis Arsenic/pharmacology Bacteria/metabolism Bacterial Physiological Phenomena Cell Movement/drug effects Chemotaxis/drug effects Electron Transport Escherichia coli/metabolism Galactose/metabolism Glucose/metabolism Lactates/metabolism Mutation Oxidative Phosphorylation Salmonella typhimurium/metabolism Succinates/metabolism Uncoupling Agents/pharmacology
Chemicals
Lactates Succinates Uncoupling Agents Adenosine Triphosphate Glucose Arsenic Galactose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Larsen S H
Adler J
Gargus J J
Hogg R W
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.4.1239
Published
1974-04-00
Pages
1239-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388200
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.4.1239 PMC Full Text View journal details
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