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PMID: 10606265 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Smaug, a novel and conserved protein, contributes to repression of nanos mRNA translation in vitro.

RNA (New York, N.Y.) ·Vol. 5 ·No. 12 ·1999-12-00 ·Pages 1535-47

Smibert CA, Lie YS, Shillinglaw W, Henzel WJ, Macdonald PM

Abstract

Proper deployment of Nanos protein at the posterior of the Drosophila embryo, where it directs posterior development, requires a combination of RNA localization and translational controls. These controls ensure that only the posteriorly-localized nanos mRNA is translated, whereas unlocalized nanos mRNA is translationally repressed. Here we describe cloning of the gene encoding Smaug, an RNA-binding protein that interacts with the sequences, SREs, in the nanos mRNA that mediate translational repression. Using an in vitro translation assay, we demonstrate that SRE-dependent repression occurs in extracts from early stage embryos. Immunodepletion of Smaug from the extracts eliminates repression, consistent with the notion that Smaug is involved. Smaug is a novel gene and the existence of potential mammalian Smaug homologs raises the possibility that Smaug represents a new class of conserved translational repressor.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Body Patterning Drosophila/embryology,genetics Drosophila Proteins Embryo, Nonmammalian/physiology Expressed Sequence Tags Gene Expression Regulation, Developmental Insect Proteins/genetics Molecular Sequence Data Morphogenesis Protein Biosynthesis RNA, Messenger/genetics,metabolism RNA-Binding Proteins/chemistry,metabolism Repressor Proteins/chemistry,metabolism Schizosaccharomyces/genetics Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Drosophila Proteins Insect Proteins RNA, Messenger RNA-Binding Proteins Repressor Proteins smg protein, Drosophila nos protein, Drosophila
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Smibert C A
Department of Biological Sciences, Stanford University, California 94305-5020, USA.
Lie Y S
Shillinglaw W
Henzel W J
Macdonald P M
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838299991392
Published
1999-12-00
Pages
1535-47
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369876
Subset
IM
Grants
NIGMS NIH HHS · GM54409 · United States
External Links
PubMed source DOI: 10.1017/s1355838299991392 PMC Full Text View journal details
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