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PMID: 3680444 Published · ppublish English Journal Article

Computer analysis of automated Edman degradation and amino acid analysis data.

Journal of chromatography ·Vol. 404 ·No. 1 ·1987-08-28 ·Pages 41-52

Henzel WJ, Rodriguez H, Watanabe C

Abstract

Computer programs are described that allow facile analysis of data from a protein sequencer and amino acid analyzer. The sequencer program provides automated sequence interpretation while requiring minimal user interaction. The program serves as a powerful aid in deciphering mixture sequences and allows routine monitoring of sequencer performance. The computer program for amino acid analysis data provides the following calculations: mole percent, protein concentration and residues per mole with comparison between theoretical and calculated values. A plot of molecular weight versus deviation from integer values is calculated providing a measure of peptide or protein purity.

MeSH Terms
Amino Acids/analysis Autoanalysis Molecular Weight Software
Chemicals
Amino Acids
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Henzel W J
Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080.
Rodriguez H
Watanabe C
Article Info
Journal
Journal of chromatography
Abbr.
J Chromatogr
Published
1987-08-28
Pages
41-52
Language
English
Region
Netherlands
NLM ID
0427043
Subset
IM
Analysis Services
Analysis Services

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