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PMID: 8114721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The deadenylation conferred by the 3' untranslated region of a developmentally controlled mRNA in Xenopus embryos is switched to polyadenylation by deletion of a short sequence element.

Molecular and cellular biology ·Vol. 14 ·No. 3 ·1994-03-00 ·Pages 1893-900

Bouvet P, Omilli F, Arlot-Bonnemains Y, Legagneux V, Roghi C, Bassez T, Osborne HB

Abstract

The maternal Xenopus Eg mRNAs are adenylated and translated in the mature oocyte and then, after fertilization, are deadenylated and released from polysomes. Therefore, after fertilization, a change occurs in the cellular mechanisms that control mRNA adenylation. In the study reported here, we show that the 3' untranslated region of Eg2 mRNA contains a cis-acting element that is required for the deadenylation of chimeric RNAs after fertilization. This cis-acting element is contained within a single 17-nucleotide portion of the Eg2 mRNA. Disruption of this deadenylation element allows adenylation of the chimeric transcripts in the embryo. Therefore, this cis-acting element is part of the sequence information required for the developmental switch from adenylation to deadenylation of the maternal Eg2 mRNA in Xenopus embryos.

Related Genes

Eg2

MeSH Terms

Animals Base Sequence Fertilization Gene Expression Regulation Molecular Sequence Data Oocytes/physiology Poly A/metabolism RNA Processing, Post-Transcriptional RNA, Messenger/genetics RNA-Binding Proteins/metabolism Recombinant Fusion Proteins/genetics Sequence Deletion Structure-Activity Relationship Xenopus laevis/embryology,genetics

Chemicals

RNA, Messenger RNA-Binding Proteins Recombinant Fusion Proteins Poly A

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Bouvet P

Department de Biologie et Génétique du Développement, URA 256 CNRS, Université de Rennes, France.

Omilli F

Arlot-Bonnemains Y

Legagneux V

Roghi C

Bassez T

Osborne H B

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33 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.14.3.1893-1900.1994

Published

1994-03-00

Pages

1893-900

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC358547

Subset

Databases

GENBANK

Z24453

External Links

PubMed source DOI: 10.1128/mcb.14.3.1893-1900.1994 PMC Full Text View journal details

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The deadenylation conferred by the 3' untranslated region of a developmentally controlled mRNA in Xenopus embryos is switched to polyadenylation by deletion of a short sequence element.

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