Home LiteratureArticle Details
PMID: 8097587 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-resolution analysis of gro alpha mRNA poly(A) shortening: regulation by interleukin-1 beta.

Nucleic acids research ·Vol. 21 ·No. 7 ·1993-04-11 ·Pages 1613-7

Stoeckle MY, Guan L

Abstract

We have previously shown that destabilization of gro alpha mRNA is associated with poly(A) shortening. In this study, we used high-resolution Northern blots to determine the rate and extent of gro alpha mRNA poly(A) shortening. gro alpha mRNA was found to undergo complete deadenylation within 2 h following withdrawal of IL-1. However, the process was not uniform: at 1 h following IL-1 withdrawal, gro alpha mRNA poly(A) lengths ranged from 0 to 180 nucleotides. There was an accumulation of deadenylated gro alpha mRNA which suggested that there may be another step before the mRNA is destroyed. Cycloheximide was found to block gro alpha mRNA degradation at the level of poly(A) shortening. Northern blots revealed a previously unrecognized periodic distribution of poly(A) lengths that was consistent with endonucleolytic cleavage between complexes of poly(A)-binding protein. The findings indicate that the degradation pathway of gro alpha mRNA is a slower version of the c-fos mRNA model, with the important additional feature that deadenylation and degradation are subject to physiologic regulation. This study provides a detailed picture of gro alpha mRNA poly(A) shortening and establishes a basis for further investigation of the mechanism by which IL-1 stabilizes specific mRNAs.

Related Genes
gro&agr
MeSH Terms
Base Sequence Blotting, Northern Cells, Cultured Chemokine CXCL1 Chemokines, CXC Chemotactic Factors/genetics Cycloheximide/pharmacology Electrophoresis, Polyacrylamide Gel Growth Substances/genetics Humans Intercellular Signaling Peptides and Proteins Interleukin-1/physiology Molecular Sequence Data Poly A/metabolism RNA Processing, Post-Transcriptional/drug effects RNA, Messenger/metabolism Ribonuclease H
Chemicals
CXCL1 protein, human Chemokine CXCL1 Chemokines, CXC Chemotactic Factors Growth Substances Intercellular Signaling Peptides and Proteins Interleukin-1 RNA, Messenger Poly A Cycloheximide Ribonuclease H
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stoeckle M Y
Division of Infectious Diseases, Cornell University Medical College, New York, NY 10021.
Guan L
References (22)
22 references, click to expand
  1. Repeating structure of cytoplasmic poly(A)-ribonucleoprotein.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1890-2 PMID: 6929525
  2. The AU-rich sequences in the 3' untranslated region mediate the increased turnover of interferon mRNA induced by glucocorticoids.
    J Exp Med. 1991 Feb 1;173(2):349-55 PMID: 1988537
  3. The Role of the poly(A) sequence in mammalian messenger RNA.
    CRC Crit Rev Biochem. 1981;10(1):1-38 PMID: 6111419
  4. The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein.
    J Cell Biol. 1983 Mar;96(3):717-21 PMID: 6833379
  5. Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.
    Nucleic Acids Res. 1985 Apr 11;13(7):2485-502 PMID: 2987855
  6. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7188-92 PMID: 2890161
  7. Poly(A) shortening and degradation of the 3' A+U-rich sequences of human c-myc mRNA in a cell-free system.
    Mol Cell Biol. 1988 Apr;8(4):1697-708 PMID: 3380094
  8. Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to beta-thromboglobulin.
    EMBO J. 1988 Jul;7(7):2025-33 PMID: 2970963
  9. Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences.
    Nature. 1988 Nov 24;336(6197):396-9 PMID: 3194021
  10. The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways.
    Genes Dev. 1989 Jan;3(1):60-72 PMID: 2496006
  11. The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation.
    Cell. 1989 Sep 8;58(5):857-67 PMID: 2673535
  12. Poly(A), poly(A) binding protein and the regulation of mRNA stability.
    Trends Biochem Sci. 1989 Sep;14(9):373-7 PMID: 2688202
  13. Processing of 9E3 mRNA and regulation of its stability in normal and Rous sarcoma virus-transformed cells.
    Mol Cell Biol. 1989 Nov;9(11):4738-45 PMID: 2557539
  14. A phorbol ester-regulated ribonuclease system controlling transforming growth factor beta 1 gene expression in hematopoietic cells.
    Mol Cell Biol. 1990 Nov;10(11):5983-90 PMID: 2233728
  15. Poly(A) tail shortening is the translation-dependent step in c-myc mRNA degradation.
    Mol Cell Biol. 1990 Dec;10(12):6132-40 PMID: 1701014
  16. Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.
    Genes Dev. 1991 Feb;5(2):221-31 PMID: 1899842
  17. Post-transcriptional regulation of gro alpha, beta, gamma, and IL-8 mRNAs by IL-1 beta.
    Nucleic Acids Res. 1991 Feb 25;19(4):917-20 PMID: 2017372
  18. IL-1 alpha induces granulocyte-macrophage colony-stimulating factor gene expression in murine B lymphocyte cell lines via mRNA stabilization.
    J Immunol. 1991 Jun 15;146(12):4190-6 PMID: 2040796
  19. An NF-kappa B-like transcription factor mediates IL-1/TNF-alpha induction of gro in human fibroblasts.
    J Immunol. 1991 Jul 15;147(2):520-7 PMID: 1906501
  20. Removal of a 3' non-coding sequence is an initial step in degradation of gro alpha mRNA and is regulated by interleukin-1.
    Nucleic Acids Res. 1992 Mar 11;20(5):1123-7 PMID: 1549476
  21. Translation initiation requires the PAB-dependent poly(A) ribonuclease in yeast.
    Cell. 1992 Sep 18;70(6):961-73 PMID: 1339314
  22. Regulation of the stability of poly(I)xpoly(C)-induced human fibroblast interferon mRNA: selective inactivation of interferon mRNA and lack of involvement of 2',5'-oligo(A) synthetase activation during the shutoff of interferon production.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3489-93 PMID: 6158049
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.7.1613
Published
1993-04-11
Pages
1613-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309371
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/21.7.1613 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2