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PMID: 10525529 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RAD51 and DMC1 form mixed complexes associated with mouse meiotic chromosome cores and synaptonemal complexes.

The Journal of cell biology ·Vol. 147 ·No. 2 ·1999-10-18 ·Pages 207-20

Tarsounas M, Morita T, Pearlman RE, Moens PB

Abstract

The eukaryotic RecA homologues RAD51 and DMC1 function in homology recognition and formation of joint-molecule recombination intermediates during yeast meiosis. The precise immunolocalization of these two proteins on the meiotic chromosomes of plants and animals has been complicated by their high degree of identity at the amino acid level. With antibodies that have been immunodepleted of cross-reactive epitopes, we demonstrate that RAD51 and DMC1 have identical distribution patterns in extracts of mouse spermatocytes in successive prophase I stages, suggesting coordinate functionality. Immunofluorescence and immunoelectron microscopy with these antibodies demonstrate colocalization of the two proteins on the meiotic chromosome cores at early prophase I. We also show that mouse RAD51 and DMC1 establish protein-protein interactions with each other and with the chromosome core component COR1(SCP3) in a two-hybrid system and in vitro binding analyses. These results suggest that the formation of a multiprotein recombination complex associated with the meiotic chromosome cores is essential for the development and fulfillment of the meiotic recombination process.

MeSH Terms
Adenosine Triphosphatases Animals Cell Cycle Proteins DNA-Binding Proteins/genetics Immunohistochemistry Male Meiosis/genetics Mice Nuclear Proteins Phosphate-Binding Proteins Rad51 Recombinase Recombination, Genetic Spermatocytes/cytology,physiology
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Dmc1 protein, mouse Nuclear Proteins Phosphate-Binding Proteins Rad51 Recombinase Rad51 protein, mouse Adenosine Triphosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tarsounas M
Department of Biology, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada.
Morita T
Pearlman R E
Moens P B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.147.2.207
Published
1999-10-18
Pages
207-20
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2174216
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.147.2.207 PMC Full Text View journal details
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