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PMID: 7860625 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Zip1-induced changes in synaptonemal complex structure and polycomplex assembly.

The Journal of cell biology ·Vol. 128 ·No. 4 ·1995-02-00 ·Pages 455-66

Sym M, Roeder GS

Abstract

The yeast Zip1 protein is a component of the synaptonemal complex (SC), which is an elaborate macromolecular structure found along the lengths of chromosomes during meiosis. Mutations that increase the length of the predicted coiled coil region of the Zip1 protein show that Zip1 influences the width of the SC. Overexpression of the ZIP1 gene results in the formation of two distinct types of higher order structures that are found in the nucleus, but not associated with chromatin. One of these structures resembles the polycomplexes that have been observed in many organisms and are thought to be aggregates of SC components. The second type of structure, which we have termed "networks," does not resemble any previously identified SC-related structure. Assembly of both polycomplexes and networks can occur independently of the Hop1 or Red1 protein, which are thought to be SC components. Our results demonstrate that Zip1 is a structural component of the central region of the SC. More specifically, we speculate that Zip1 is a component of the transverse filaments that lie perpendicular to the long axis of the complex.

MeSH Terms
Chromosomes, Fungal/ultrastructure DNA Mutational Analysis DNA-Binding Proteins/physiology Double-Blind Method Fungal Proteins/genetics,isolation & purification,physiology,ultrastructure Immunohistochemistry Microscopy, Electron Nuclear Proteins Protein Structure, Tertiary Recombinant Proteins/isolation & purification,ultrastructure Saccharomyces cerevisiae/genetics,growth & development,ultrastructure Saccharomyces cerevisiae Proteins Spores, Fungal/growth & development Structure-Activity Relationship Synaptonemal Complex/genetics,physiology
Chemicals
DNA-Binding Proteins Fungal Proteins HOP1 protein, S cerevisiae Nuclear Proteins RED1 protein, S cerevisiae Recombinant Proteins Saccharomyces cerevisiae Proteins Zip1 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sym M
Department of Biology, Yale University, New Haven, Connecticut 06520-8103.
Roeder G S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.128.4.455
Published
1995-02-00
Pages
455-66
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2199901
Subset
IM
Grants
NIGMS NIH HHS · 2 T32 GM07223-18 · United States
NIGMS NIH HHS · GM28904 · United States
External Links
PubMed source DOI: 10.1083/jcb.128.4.455 PMC Full Text View journal details
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