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PMID: 9611194 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Meiotic chromosome morphology and behavior in zip1 mutants of Saccharomyces cerevisiae.

Genetics ·Vol. 149 ·No. 2 ·1998-06-00 ·Pages 817-32

Tung KS, Roeder GS

Abstract

The yeast Zip1 protein (Zip1p) is a component of the central region of the synaptonemal complex (SC). Zip1p is predicted to form a dimer consisting of a coiled-coil domain flanked by globular domains. To analyze the organization of Zip1p within the SC, in-frame deletions of ZIP1 were constructed and analyzed. The results demonstrate that the C terminus but not the N terminus of Zip1p is required for its localization to chromosomes. Deletions in the carboxy half of the predicted coiled-coil region cause decreases in the width of the SC. Based on these results, a model for the organization of Zip1p within the SC is proposed. zip1 deletion mutations were also examined for their effects on sporulation, spore viability, crossing over, and crossover interference. The results demonstrate that the extent of synapsis is positively correlated with the levels of spore viability, crossing over, and crossover interference. In contrast, the role of Zip1p in synapsis is separable from its role in meiotic cell cycle progression. zip1 mutants display interval-specific effects on crossing over.

MeSH Terms
Antibodies, Fungal Chromosomes, Fungal/chemistry,genetics,immunology,metabolism Crossing Over, Genetic/genetics Fungal Proteins/genetics,immunology,metabolism Meiosis/genetics Mutagenesis, Site-Directed Nuclear Proteins Protein Structure, Secondary Protein Structure, Tertiary Reading Frames/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Spores, Fungal/genetics Staining and Labeling Synaptonemal Complex/genetics
Chemicals
Antibodies, Fungal Fungal Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins Zip1 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tung K S
Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06520-8103, USA.
Roeder G S
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/149.2.817
Published
1998-06-00
Pages
817-32
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460213
Subset
IM
Grants
NIGMS NIH HHS · GM28904 · United States
External Links
PubMed source DOI: 10.1093/genetics/149.2.817 PMC Full Text View journal details
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