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PMID: 10377429 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Efficient export of the glucose transporter Hxt1p from the endoplasmic reticulum requires Gsf2p.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 13 ·1999-06-22 ·Pages 7415-20

Sherwood PW, Carlson M

Abstract

Mutations in the GSF2 gene cause glucose starvation phenotypes in Saccharomyces cerevisiae. We have isolated the HXT1 gene, which encodes a low-affinity, high-capacity glucose transporter, as a multicopy suppressor of a gsf2 mutation. We show that gsf2 mutants accumulate Hxt1p in the endoplasmic reticulum (ER) and that Gsf2p is a 46-kDa integral membrane protein localized to the ER. gsf2 mutants also display a galactose growth defect and abnormal localization of the galactose transporter Gal2p but are not defective in function or localization of the high-affinity glucose transporter Hxt2p. These findings suggest that Gsf2p functions in the ER to promote the secretion of certain hexose transporters.

MeSH Terms
Biological Transport/genetics Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Fungal Glucose/metabolism Glucose Transport Proteins, Facilitative Membrane Proteins/genetics,metabolism Monosaccharide Transport Proteins/genetics,metabolism Mutation Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Fungal Proteins Glucose Transport Proteins, Facilitative Gsf2 protein, S cerevisiae HXT1 protein, S cerevisiae Membrane Proteins Monosaccharide Transport Proteins Saccharomyces cerevisiae Proteins Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sherwood P W
Department of Genetics and Development, Columbia University, New York, NY 10032, USA.
Carlson M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.13.7415
Published
1999-06-22
Pages
7415-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22100
Subset
IM
Grants
NIGMS NIH HHS · R01 GM034095 · United States
NIGMS NIH HHS · R37 GM034095 · United States
NIGMS NIH HHS · GM34095 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.13.7415 PMC Full Text View journal details
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