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PMID: 7935396 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The GLC7 type 1 protein phosphatase is required for glucose repression in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 14 ·No. 10 ·1994-10-00 ·Pages 6789-96

Tu J, Carlson M

Abstract

We cloned the GLC7/DIS2S1 gene by complementation of the cid1-226 mutation, which relieves glucose repression in Saccharomyces cerevisiae. GLC7 encodes the catalytic subunit of type 1 protein phosphatase (PP1). Genetic analysis and sequencing showed that cid1-226 is an allele of GLC7, now designated glc7-T152K, which alters threonine 152 to lysine. We also show that the glc7-1 and glc7-T152K alleles cause distinct phenotypes: glc7-1 causes a severe defect in glycogen accumulation but does not relieve glucose repression, whereas glc7-T152K does not prevent glycogen accumulation. These findings are discussed in light of evidence that interaction with different regulatory or targeting subunits directs the participation of PP1 in diverse cellular regulatory mechanisms. Finally, genetic studies suggest that PP1 functions antagonistically to the SNF1 protein kinase in the regulatory response to glucose.

Related Genes
CID1
MeSH Terms
Alleles Base Sequence Cloning, Molecular Fungal Proteins/genetics Gene Expression Regulation, Fungal/drug effects Genetic Complementation Test Glucose/pharmacology Glycogen/biosynthesis Glycoside Hydrolases/biosynthesis Molecular Sequence Data Mutation Phenotype Phosphoprotein Phosphatases/genetics Protein Serine-Threonine Kinases/antagonists & inhibitors Saccharomyces cerevisiae/genetics Sequence Analysis, DNA Spores, Fungal/growth & development Trehalose/biosynthesis beta-Fructofuranosidase
Chemicals
Fungal Proteins Glycogen Trehalose SNF1-related protein kinases Protein Serine-Threonine Kinases Phosphoprotein Phosphatases Glycoside Hydrolases beta-Fructofuranosidase Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tu J
Institute of Cancer Research, Columbia University, New York, New York 10032.
Carlson M
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.10.6789-6796.1994
Published
1994-10-00
Pages
6789-96
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359209
Subset
IM
Grants
NIGMS NIH HHS · GM34095 · United States
External Links
PubMed source DOI: 10.1128/mcb.14.10.6789-6796.1994 PMC Full Text View journal details
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