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PMID: 10377410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cdc20 associates with the kinase aurora2/Aik.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 13 ·1999-06-22 ·Pages 7306-11

Farruggio DC, Townsley FM, Ruderman JV

Abstract

Cdc20/fizzy family proteins are involved in activation of the anaphase-promoting complex/cyclosome, which catalyzes the ubiquitin-dependent proteolysis of cell cycle regulatory proteins such as anaphase inhibitors and mitotic cyclins, leading to chromosome segregation and exit from mitosis. Previous work has shown that human Cdc20 (hCdc20/p55CDC) associates with one or more kinases. We report here that Cdc20-associated myelin basic protein kinase activity peaks sharply in early M phase (embryonic cells) or in G2 phase (somatic cells). In HeLa cells, Cdc20 is associated with the kinase aurora2/Aik. Aurora2/Aik is a member of the aurora/Ipl1 family of kinases that, like Cdc20, previously has been shown to be localized at mitotic spindle poles and is involved in regulating chromosome segregation and maintaining genomic stability. The demonstration that Cdc20 is associated with aurora2/Aik suggests that some function of Cdc20 is carried out or regulated through its association with aurora2/Aik.

MeSH Terms
Aurora Kinases Cdc20 Proteins Cell Cycle/physiology Cell Cycle Proteins/physiology Female Fertilization HeLa Cells Humans Molecular Sequence Data Oocytes Protein Kinases/physiology Protein Serine-Threonine Kinases Saccharomyces cerevisiae Proteins Signal Transduction
Chemicals
CDC20 protein, S cerevisiae Cdc20 Proteins Cell Cycle Proteins Saccharomyces cerevisiae Proteins Protein Kinases Aurora Kinases Protein Serine-Threonine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Farruggio D C
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Townsley F M
Ruderman J V
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.13.7306
Published
1999-06-22
Pages
7306-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22081
Subset
IM
Grants
Wellcome Trust · United Kingdom
NICHD NIH HHS · R01 HD023696 · United States
NICHD NIH HHS · HD23696 · United States
Databases
GENBANK
AF052575
External Links
PubMed source DOI: 10.1073/pnas.96.13.7306 PMC Full Text View journal details
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