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PMID: 1922065 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes.

Molecular and cellular biology ·Vol. 11 ·No. 11 ·1991-11-00 ·Pages 5592-602

Sethi N, Monteagudo MC, Koshland D, Hogan E, Burke DJ

Abstract

Previous analysis of cdc20 mutants of the yeast Saccharomyces cerevisiae suggests that the CDC20 gene product (Cdc20p) is required for two microtubule-dependent processes, nuclear movements prior to anaphase and chromosome separation. Here we report that cdc20 mutants are defective for a third microtubule-mediated event, nuclear fusion during mating of G1 cells, but appear normal for a fourth microtubule-dependent process, nuclear migration after DNA replication. Therefore, Cdc20p is required for a subset of microtubule-dependent processes and functions at multiple stages in the life cycle. Consistent with this interpretation, we find that cdc20 cells arrested by alpha-factor or at the restrictive temperature accumulate anomalous microtubule structures, as detected by indirect immunofluorescence. The anomalous microtubule staining patterns are due to cdc20 because intragenic revertants that revert the temperature sensitivity have normal microtubule morphologies. cdc20 mutants have a sevenfold increase in the intensity of antitubulin fluorescence in intranuclear spindles compared with spindles from wild-type cells, yet the total amount of tubulin is indistinguishable by Western immunoblot analysis. This result suggests that Cdc20p modulates microtubule structure in wild-type cells either by promoting microtubule disassembly or by altering the surface of the microtubules. Finally, we cloned and sequenced CDC20 and show that it encodes a member of a family of proteins that share homology to the beta subunit of transducin.

MeSH Terms
Amino Acid Sequence Base Sequence Crosses, Genetic DNA Repair DNA, Fungal/genetics Fluorescent Antibody Technique Fungal Proteins/genetics Genes, Fungal Genotype Microtubules/physiology Molecular Sequence Data Plasmids Restriction Mapping Saccharomyces cerevisiae/genetics,physiology Sequence Homology, Nucleic Acid Transducin/genetics Tubulin/analysis,genetics
Chemicals
DNA, Fungal Fungal Proteins Tubulin Transducin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sethi N
Department of Biology, University of Virginia, Charlottesville 22901.
Monteagudo M C
Koshland D
Hogan E
Burke D J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.11.5592-5602.1991
Published
1991-11-00
Pages
5592-602
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361930
Subset
IM
Grants
NIGMS NIH HHS · GM 40334-02 · United States
Databases
GENBANK
M64827, S43620, S58910, S59957, S59963, S59966, S59969, S59977, S59984, X59428
External Links
PubMed source DOI: 10.1128/mcb.11.11.5592-5602.1991 PMC Full Text View journal details
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