Location of the regions recognized by five commercial antibodies on the tubulin molecule.

Through limited proteolysis of the tubulin molecule with trypsin, chymotrypsin, subtilisin, or pronase we have mapped the regions recognized by five commercial antibodies. Two of them recognized a sequence between amino acids 340 and 400 near the C terminal of the alpha or beta subunits, one recognized a sequence between amino acids 120 and 150 present in both subunits, and another one probably recognized a conformational epitope. Simultaneously we have confirmed the results obtained for other antibodies which recognized a region previously mapped on the tubulin molecule.