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PMID: 9649427 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.

The EMBO journal ·Vol. 17 ·No. 13 ·1998-07-01 ·Pages 3565-75

Lorca T, Castro A, Martinez AM, Vigneron S, Morin N, Sigrist S, Lehner C, Dorée M, Labbé JC

Abstract

The Xenopus homologue of Drosophila Fizzy and budding yeast CDC20 has been characterized. The encoded protein (X-FZY) is a component of a high molecular weight complex distinct from the APC/cyclosome. Antibodies directed against FZY were produced and shown to prevent calmodulin-dependent protein kinase II (CaMKII) from inducing the metaphase to anaphase transition of spindles assembled in vitro in Xenopus egg extracts, and this was associated with suppression of the degradation of mitotic cyclins. The same antibodies suppressed M phase-promoting factor (MPF)-dependent activation of the APC/cyclosome in interphase egg extracts, although they did not appear to alter the pattern or extent of MPF-dependent phosphorylation of APC/cyclosome subunits. As these phosphorylations are thought to be essential for APC/cyclosome activation in eggs and early embryos, we conclude that at least two events are required for MPF to activate the APC/cyclosome, allowing both chromatid segregation and full degradation of mitotic cyclins. The first one, which does not require FZY function, is the phosphorylation of APC/cyclosome subunits. The second one, that requires FZY function (even in the absence of MAD2 protein and when the spindle assembly checkpoint is not activated) is not yet understood at its molecular level.

MeSH Terms
Amino Acid Sequence Anaphase-Promoting Complex-Cyclosome Animals Antibodies/immunology Base Sequence Calcium-Binding Proteins Carrier Proteins Cdc20 Proteins Cell Cycle Proteins/genetics,metabolism DNA, Complementary Enzyme Activation Fungal Proteins Humans Ligases/metabolism Maturation-Promoting Factor/metabolism Molecular Sequence Data Nuclear Proteins Ovum Rabbits Sequence Homology, Amino Acid Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases Xenopus Xenopus Proteins
Chemicals
Antibodies Calcium-Binding Proteins Carrier Proteins Cdc20 Proteins Cdc20 protein, Xenopus Cell Cycle Proteins DNA, Complementary Fungal Proteins Nuclear Proteins Xenopus Proteins CDC20 protein, human Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome Ubiquitin-Protein Ligases Maturation-Promoting Factor Ligases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Lorca T
Centre de Recherches de Biochimie Macromoléculaire, CNRS UPR 1086, 1919 route de Mende, 34293 Montpellier Cedex 5, France.
Castro A
Martinez A M
Vigneron S
Morin N
Sigrist S
Lehner C
Dorée M
Labbé J C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.13.3565
Published
1998-07-01
Pages
3565-75
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170693
Subset
IM
Databases
GENBANK
AF034578
External Links
PubMed source DOI: 10.1093/emboj/17.13.3565 PMC Full Text View journal details
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