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PMID: 9852151 Published · ppublish English Journal Article

Role of xklp3, a subunit of the Xenopus kinesin II heterotrimeric complex, in membrane transport between the endoplasmic reticulum and the Golgi apparatus.

The Journal of cell biology ·Vol. 143 ·No. 6 ·1998-12-14 ·Pages 1559-73

Le Bot N, Antony C, White J, Karsenti E, Vernos I

Abstract

The function of the Golgi apparatus is to modify proteins and lipids synthesized in the ER and sort them to their final destination. The steady-state size and function of the Golgi apparatus is maintained through the recycling of some components back to the ER. Several lines of evidence indicate that the spatial segregation between the ER and the Golgi apparatus as well as trafficking between these two compartments require both microtubules and motors. We have cloned and characterized a new Xenopus kinesin like protein, Xklp3, a subunit of the heterotrimeric Kinesin II. By immunofluorescence it is found in the Golgi region. A more detailed analysis by EM shows that it is associated with a subset of membranes that contain the KDEL receptor and are localized between the ER and Golgi apparatus. An association of Xklp3 with the recycling compartment is further supported by a biochemical analysis and the behavior of Xklp3 in BFA-treated cells. The function of Xklp3 was analyzed by transfecting cells with a dominant-negative form lacking the motor domain. In these cells, the normal delivery of newly synthesized proteins to the Golgi apparatus is blocked. Taken together, these results indicate that Xklp3 is involved in the transport of tubular-vesicular elements between the ER and the Golgi apparatus.

MeSH Terms
Amino Acid Sequence Animals Calcium-Binding Proteins/chemistry,genetics,physiology Cell Line Endoplasmic Reticulum/physiology Golgi Apparatus/physiology Green Fluorescent Proteins Kinesins/physiology Luminescent Proteins/metabolism Macromolecular Substances Microscopy, Immunoelectron Molecular Sequence Data Muscle Proteins/chemistry,genetics,physiology Oocytes/physiology Polymerase Chain Reaction Receptors, Peptide/chemistry,physiology Recombinant Fusion Proteins/chemistry,metabolism Transfection Xenopus Xenopus Proteins
Chemicals
Calcium-Binding Proteins KDEL receptor Luminescent Proteins Macromolecular Substances Muscle Proteins Receptors, Peptide Recombinant Fusion Proteins XKLP3 protein, Xenopus Xenopus Proteins kinesin-II Green Fluorescent Proteins Kinesins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Le Bot N
Cell Biology and Biophysics Program, European Molecular Biological Laboratory, D-69117 Heidelberg, Germany.
Antony C
White J
Karsenti E
Vernos I
References (55)
55 references, click to expand
  1. Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles.
    J Cell Biol. 1996 Apr;133(1):1-4 PMID: 8601599
  2. Interaction of a Golgi-associated kinesin-like protein with Rab6.
    Science. 1998 Jan 23;279(5350):580-5 PMID: 9438855
  3. Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of KIF3A/3B.
    Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8443-8 PMID: 8710890
  4. The length of secondary chromosomal constrictions in normal individuals and in a nucleolar mutant of Xenopus laevis.
    Cytogenetics. 1969;8(6):427-38 PMID: 5365248
  5. Comparison of in vivo and in vitro ribosomal RNA synthesis in nucleolar mutants of Xenopus laevis.
    In Vitro. 1977 Sep;13(9):557-63 PMID: 562840
  6. Cytochemical localization of terminal N-acetyl-D-galactosamine residues in cellular compartments of intestinal goblet cells: implications for the topology of O-glycosylation.
    J Cell Biol. 1984 Feb;98(2):399-406 PMID: 6693488
  7. Direct visualization of protein transport and processing in the living cell by microinjection of specific antibodies.
    Cell. 1984 Nov;39(1):99-109 PMID: 6091920
  8. Localization of binding sites for concanavalin A, Ricinus communis I and Helix pomatia lectin in the Golgi apparatus of rat small intestinal absorptive cells.
    J Histochem Cytochem. 1985 Sep;33(9):905-14 PMID: 4020101
  9. Oligomerization is essential for transport of vesicular stomatitis viral glycoprotein to the cell surface.
    Cell. 1986 Sep 12;46(6):929-37 PMID: 3019557
  10. Characterization of the KIF3C neural kinesin-like motor from mouse.
    Mol Biol Cell. 1998 Feb;9(2):249-61 PMID: 9450952
  11. Cytoskeletal proteins and Golgi dynamics.
    Curr Opin Cell Biol. 1998 Feb;10(1):52-9 PMID: 9484595
  12. Golgi vesiculation and lysosome dispersion in cells lacking cytoplasmic dynein.
    J Cell Biol. 1998 Apr 6;141(1):51-9 PMID: 9531547
  13. A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT).
    J Cell Biol. 1998 May 18;141(4):979-92 PMID: 9585416
  14. Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.
    J Cell Biol. 1998 May 18;141(4):993-1008 PMID: 9585417
  15. Characterization of KIF1C, a new kinesin-like protein involved in vesicle transport from the Golgi apparatus to the endoplasmic reticulum.
    J Biol Chem. 1998 Aug 7;273(32):20267-75 PMID: 9685376
  16. Unravelling Golgi membrane traffic with green fluorescent protein chimeras.
    Trends Cell Biol. 1998 Jan;8(1):16-20 PMID: 9695802
  17. Heterotrimeric kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores.
    J Cell Biol. 1998 Dec 14;143(6):1547-58 PMID: 9852150
  18. Molecular cloning, characterization, subcellular localization and dynamics of p23, the mammalian KDEL receptor.
    J Cell Biol. 1993 Jan;120(2):325-38 PMID: 8380600
  19. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  20. Condensation-sorting events in the rough endoplasmic reticulum of exocrine pancreatic cells.
    J Cell Biol. 1989 Jul;109(1):35-50 PMID: 2745555
  21. Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum.
    J Cell Biol. 1989 Jul;109(1):61-72 PMID: 2745557
  22. Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum.
    Cell. 1989 Aug 25;58(4):707-18 PMID: 2527615
  23. Immuno-localization of the insulin regulatable glucose transporter in brown adipose tissue of the rat.
    J Cell Biol. 1991 Apr;113(1):123-35 PMID: 2007617
  24. Predicting coiled coils from protein sequences.
    Science. 1991 May 24;252(5009):1162-4 PMID: 2031185
  25. Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum.
    Cell. 1992 Jan 24;68(2):353-64 PMID: 1310258
  26. Cytoplasmic dynein participates in the centrosomal localization of the Golgi complex.
    J Cell Biol. 1992 Sep;118(6):1333-45 PMID: 1387874
  27. Multiple kinesin-like transcripts in Xenopus oocytes.
    Dev Biol. 1993 May;157(1):232-9 PMID: 8482413
  28. Novel heterotrimeric kinesin-related protein purified from sea urchin eggs.
    Nature. 1993 Nov 18;366(6452):268-70 PMID: 8232586
  29. KIF3A is a new microtubule-based anterograde motor in the nerve axon.
    J Cell Biol. 1994 Jun;125(5):1095-107 PMID: 7515068
  30. Kinesin-mediated organelle translocation revealed by specific cellular manipulations.
    J Cell Biol. 1994 Nov;127(4):1021-39 PMID: 7962067
  31. Characterization of the KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport.
    J Cell Biol. 1994 Nov;127(4):1041-8 PMID: 7525600
  32. Localization of the Lys, Asp, Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate compartment in mammalian cells.
    J Cell Biol. 1994 Dec;127(6 Pt 1):1557-74 PMID: 7798312
  33. Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic.
    J Cell Biol. 1995 Feb;128(3):293-306 PMID: 7844144
  34. Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to the external environment.
    J Mol Biol. 1995 Mar 31;247(3):377-89 PMID: 7714894
  35. Xklp1, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning.
    Cell. 1995 Apr 7;81(1):117-27 PMID: 7720067
  36. Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP85/95.
    J Mol Biol. 1995 Sep 15;252(2):157-62 PMID: 7674298
  37. Immunolocalization of the heterotrimeric kinesin-related protein KRP(85/95) in the mitotic apparatus of sea urchin embryos.
    Dev Biol. 1995 Sep;171(1):182-94 PMID: 7556895
  38. KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport.
    J Cell Biol. 1995 Sep;130(6):1387-99 PMID: 7559760
  39. The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane.
    J Cell Biol. 1995 Dec;131(6 Pt 1):1517-27 PMID: 8522608
  40. Differential response of resident proteins and cycling proteins of the Golgi to brefeldin A.
    Eur J Cell Biol. 1995 Oct;68(2):199-205 PMID: 8575466
  41. XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.
    Cell. 1996 Jan 12;84(1):37-47 PMID: 8548824
  42. Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis.
    Cell. 1996 Jan 12;84(1):49-59 PMID: 8548825
  43. Improving structural integrity of cryosections for immunogold labeling.
    Histochem Cell Biol. 1996 Jul;106(1):41-58 PMID: 8858366
  44. SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase.
    J Biol Chem. 1996 Oct 25;271(43):27013-7 PMID: 8900189
  45. The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles.
    J Cell Sci. 1996 Mar;109 ( Pt 3):541-9 PMID: 8907700
  46. Movement of nuclei along microtubules in Xenopus egg extracts.
    Curr Biol. 1997 Mar 1;7(3):211-4 PMID: 9395409
  47. Characterization of brefeldin A induced vesicular structures containing cycling proteins of the intermediate compartment/cis-Golgi network.
    FEBS Lett. 1997 Mar 3;404(1):75-81 PMID: 9074641
  48. Regulated bidirectional motility of melanophore pigment granules along microtubules in vitro.
    Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3720-5 PMID: 9108044
  49. Dissociation of coatomer from membranes is required for brefeldin A-induced transfer of Golgi enzymes to the endoplasmic reticulum.
    J Cell Biol. 1997 Apr 21;137(2):319-33 PMID: 9128245
  50. Partitioning of the Golgi apparatus during mitosis in living HeLa cells.
    J Cell Biol. 1997 Jun 16;137(6):1211-28 PMID: 9182657
  51. Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos.
    J Cell Biol. 1997 Sep 8;138(5):1009-22 PMID: 9281580
  52. ER-to-Golgi transport visualized in living cells.
    Nature. 1997 Sep 4;389(6646):81-5 PMID: 9288971
  53. Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution.
    J Cell Biol. 1997 Oct 20;139(2):469-84 PMID: 9334349
  54. Golgi tubule traffic and the effects of brefeldin A visualized in living cells.
    J Cell Biol. 1997 Dec 1;139(5):1137-55 PMID: 9382862
  55. Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex.
    J Cell Biol. 1996 Feb;132(3):371-80 PMID: 8636215
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.143.6.1559
Published
1998-12-14
Pages
1559-73
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132969
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.143.6.1559 PMC Full Text View journal details
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