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PMID: 7962067 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Kinesin-mediated organelle translocation revealed by specific cellular manipulations.

The Journal of cell biology ·Vol. 127 ·No. 4 ·1994-11-00 ·Pages 1021-39

Feiguin F, Ferreira A, Kosik KS, Caceres A

Abstract

The distribution of membrane-bound organelles was studied in cultured hippocampal neurons after antisense oligonucleotide suppression of the kinesin-heavy chain (KHC). We observed reduced 3,3'-dihexyloxacarbocyanine iodide (DiOC6(3)) fluorescent staining in neurites and growth cones. In astrocytes, KHC suppression results in the disappearance of the DiOC6(3)-positive reticular network from the cell periphery, and a parallel accumulation of label within the cell center. On the other hand, mitochondria microtubules and microfilaments display a distribution that closely resembles that observed in control cells. KHC suppression of neurons and astrocytes completely inhibited the Brefeldin A-induced spreading and tubulation of the Golgi-associated structure enriched in mannose-6-phosphate receptors. In addition, KHC suppression prevents the low pH-induced anterograde redistribution of late endocytic structures. Taken collectively, these observations suggest that in living neurons, kinesin mediates the anterograde transport of tubulovesicular structures originated in the central vacuolar system (e.g., the endoplasmic reticulum) and that the regulation of kinesin-membrane interactions may be of key importance for determining the intracellular distribution of selected organelles.

MeSH Terms
Actin Cytoskeleton/drug effects,physiology,ultrastructure Animals Base Sequence Brefeldin A Carbocyanines Cells, Cultured Cyclopentanes/pharmacology Endocytosis/drug effects,physiology Fluorescent Dyes Golgi Apparatus/physiology,ultrastructure Hippocampus/physiology Kinesins/antagonists & inhibitors,genetics,physiology Microtubules/drug effects,physiology,ultrastructure Mitochondria/drug effects,physiology,ultrastructure Molecular Sequence Data Neurites/drug effects,physiology,ultrastructure Oligonucleotides, Antisense/pharmacology Organelles/drug effects,physiology,ultrastructure Protein Synthesis Inhibitors/pharmacology Pyramidal Cells/drug effects,physiology,ultrastructure Rhodamine 123 Rhodamines
Chemicals
Carbocyanines Cyclopentanes Fluorescent Dyes Oligonucleotides, Antisense Protein Synthesis Inhibitors Rhodamines Rhodamine 123 Brefeldin A 3,3'-dihexyl-2,2'-oxacarbocyanine Kinesins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Feiguin F
Instituto Mercedes y Martin Fereyra, Cordoba, Argentina.
Ferreira A
Kosik K S
Caceres A
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51 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.4.1021
Published
1994-11-00
Pages
1021-39
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200060
Subset
IM
Grants
FIC NIH HHS · TW00137-02 · United States
External Links
PubMed source DOI: 10.1083/jcb.127.4.1021 PMC Full Text View journal details
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