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PMID: 9813083 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

KDEL receptor (Erd2p)-mediated retrograde transport of the cholera toxin A subunit from the Golgi involves COPI, p23, and the COOH terminus of Erd2p.

The Journal of cell biology ·Vol. 143 ·No. 3 ·1998-11-02 ·Pages 601-12

Majoul I, Sohn K, Wieland FT, Pepperkok R, Pizza M, Hillemann J, Söling HD

Abstract

A cholera toxin mutant (CTX-K63) unable to raise cAMP levels was used to study in Vero cells the retrograde transport of the toxin A subunit (CTX-A-K63), which possesses a COOH-terminal KDEL retrieval signal. Microinjected GTP-gamma-S inhibits the internalization as well as Golgi-ER transport of CTX-A-K63. The appearance of CTX-A-K63 in the Golgi induces a marked dispersion of Erd2p and p53 but not of the Golgi marker giantin. Erd2p is translocated under these conditions most likely to the intermediate compartment as indicated by an increased colocalization of Erd2p with mSEC13, a member of the mammalian coat protein II complex. IgGs as well as Fab fragments directed against Erd2p, beta-COP, or p23, a new member of the p24 protein family, inhibit or block retrograde transport of CTX-A-K63 from the Golgi without affecting its internalization or its transport to the Golgi. Anti-Erd2p antibodies do not affect the binding of CTX-A to Erd2p, but inhibit the CTX-K63-induced translocation of Erd2p and p53.

MeSH Terms
Adenosine Diphosphate Ribose/metabolism Amino Acid Sequence Animals Biological Transport, Active Chlorocebus aethiops Cholera Toxin/metabolism Coatomer Protein Endoplasmic Reticulum/metabolism Golgi Apparatus/metabolism Guanosine 5'-O-(3-Thiotriphosphate)/metabolism Immunoglobulin Fab Fragments/immunology Immunoglobulin G/immunology Membrane Proteins/metabolism Microtubule-Associated Proteins/immunology,metabolism Molecular Sequence Data Mutagenesis Rabbits Receptors, Peptide/immunology,metabolism Tumor Suppressor Protein p53/metabolism Vero Cells
Chemicals
Coatomer Protein Immunoglobulin Fab Fragments Immunoglobulin G KDEL receptor Membrane Proteins Microtubule-Associated Proteins Receptors, Peptide Tumor Suppressor Protein p53 Adenosine Diphosphate Ribose Guanosine 5'-O-(3-Thiotriphosphate) Cholera Toxin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Majoul I
Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany.
Sohn K
Wieland F T
Pepperkok R
Pizza M
Hillemann J
Söling H D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.143.3.601
Published
1998-11-02
Pages
601-12
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2148140
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.143.3.601 PMC Full Text View journal details
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