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PMID: 8703076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bimodal interaction of coatomer with the p24 family of putative cargo receptors.

Science (New York, N.Y.) ·Vol. 273 ·No. 5280 ·1996-09-06 ·Pages 1396-9

Fiedler K, Veit M, Stamnes MA, Rothman JE

Abstract

Cytoplasmic domains of members of the p24 family of putative cargo receptors were shown to bind to coatomer, the coat protein of COPI-coated transport vesicles. Domains that contained dilysine endoplasmic reticulum retrieval signals bound the alpha-, beta'-, and epsilon-COP subunits of coatomer, whereas other p24 domains bound the beta-, gamma-, and zeta-COP subunits and required a phenylalanine-containing motif. Transit of a CD8-p24 chimera from the endoplasmic reticulum through the Golgi complex was slowed when the phenylalanine motif was mutated, suggesting that this motif may function as an anterograde transport signal. The either-or bimodal binding of coatomer to p24 tails suggests models for how coatomer can potentially package retrograde-directed and anterograde-directed cargo into distinct COPI-coated vesicles.

MeSH Terms

Amino Acid Sequence Animals Biological Transport CHO Cells Carrier Proteins/chemistry,metabolism Cell Membrane/metabolism Coated Vesicles/metabolism Coatomer Protein Cricetinae Endoplasmic Reticulum/metabolism Golgi Apparatus/metabolism Lysine/analysis Membrane Proteins/chemistry,metabolism Molecular Sequence Data Nuclear Envelope/metabolism Phenylalanine/analysis Recombinant Fusion Proteins/metabolism

Chemicals

Carrier Proteins Coatomer Protein Membrane Proteins Recombinant Fusion Proteins Phenylalanine Lysine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Fiedler K

Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.

Veit M

Stamnes M A

Rothman J E

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.273.5280.1396

Published

1996-09-06

Pages

1396-9

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

F06012, F07445, L22015, R14234, R25915, T11000, T17481, T27339, T27390, T32238, T34121, T36996, T46519, T48545, T48838, T98284, U00059, U26264, X53592, X67317, X87331, Z34726, Z48432, Z49810

External Links

PubMed source DOI: 10.1126/science.273.5280.1396 View journal details

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Bimodal interaction of coatomer with the p24 family of putative cargo receptors.

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