Home LiteratureArticle Details
PMID: 9765201 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The HIV-1 Tat cellular coactivator Tat-SF1 is a general transcription elongation factor.

Genes & development ·Vol. 12 ·No. 19 ·1998-10-01 ·Pages 2992-6

Li XY, Green MR

Abstract

The human immunodeficiency virus type 1 (HIV-1) Tat protein strongly and specifically stimulates transcription elongation from the HIV-1 LTR and provides an important in vitro model system to study this process. Here we use protein-affinity chromatography to identify cellular factors involved in transcription elongation. A Tat-affinity column bound one transcription factor, Tat-SF1, efficiently and selectively. Tat-SF1 was identified originally as a Tat-specific coactivator, but we show it is a general transcription elongation factor. Our results also reveal the existence of an ATP-inactivatable general elongation factor (AIEF) required for Tat-SF1 activity and for which Tat can substitute functionally.

MeSH Terms
Electrophoresis, Polyacrylamide Gel Gene Products, tat/physiology HIV Long Terminal Repeat/genetics HIV-1/genetics Humans Plasmids Trans-Activators/physiology Transcription, Genetic tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat HTATSF1 protein, human Trans-Activators tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Li X Y
Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA.
Green M R
References (35)
35 references, click to expand
  1. Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product.
    Nature. 1987 Dec 3-9;330(6147):489-93 PMID: 2825027
  2. Transcription elongation factor P-TEFb mediates Tat activation of HIV-1 transcription at multiple stages.
    EMBO J. 1998 Jul 1;17(13):3681-91 PMID: 9649438
  3. Transcription activation by the adenovirus E1a protein.
    Nature. 1989 Mar 2;338(6210):39-44 PMID: 2521923
  4. HIV-1 Tat protein increases transcriptional initiation and stabilizes elongation.
    Cell. 1989 Oct 20;59(2):283-92 PMID: 2553266
  5. HIV-1 Tat protein trans-activates transcription in vitro.
    Cell. 1990 Nov 16;63(4):791-802 PMID: 2225077
  6. The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):4045-9 PMID: 2023953
  7. The HIV-1 Tat protein activates transcription from an upstream DNA-binding site: implications for Tat function.
    Genes Dev. 1991 Dec;5(12B):2496-507 PMID: 1752440
  8. HIV-1 Tat protein promotes formation of more-processive elongation complexes.
    EMBO J. 1991 Dec;10(13):4189-96 PMID: 1756726
  9. HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors.
    Genes Dev. 1992 Apr;6(4):655-66 PMID: 1559613
  10. Does HIV-1 Tat induce a change in viral initiation rights?
    Cell. 1993 May 7;73(3):417-20 PMID: 8490957
  11. Dual role of TFIIH in DNA excision repair and in transcription by RNA polymerase II.
    Nature. 1994 Apr 21;368(6473):769-72 PMID: 8152490
  12. Control of RNA initiation and elongation at the HIV-1 promoter.
    Annu Rev Biochem. 1994;63:717-43 PMID: 7979253
  13. Novel mechanism and factor for regulation by HIV-1 Tat.
    EMBO J. 1995 Jan 16;14(2):321-8 PMID: 7835343
  14. Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor.
    J Virol. 1995 Mar;69(3):1612-20 PMID: 7853496
  15. Regulation of transcriptional elongation by RNA polymerase II.
    Curr Opin Genet Dev. 1995 Apr;5(2):210-6 PMID: 7613091
  16. Positive regulation of general transcription factor SIII by a tailed ubiquitin homolog.
    Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7172-6 PMID: 7638163
  17. Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II.
    Science. 1995 Sep 8;269(5229):1439-43 PMID: 7660129
  18. Intramolecular inhibition of activating transcription factor-2 function by its DNA-binding domain.
    Genes Dev. 1996 Mar 1;10(5):517-27 PMID: 8598283
  19. An RNA polymerase II elongation factor encoded by the human ELL gene.
    Science. 1996 Mar 29;271(5257):1873-6 PMID: 8596958
  20. Transcriptional elongation and cancer. Tumorigenesis.
    Curr Biol. 1996 Aug 1;6(8):943-4 PMID: 8805321
  21. Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat.
    Science. 1996 Oct 25;274(5287):605-10 PMID: 8849451
  22. The RNA polymerase II general elongation factors.
    Trends Biochem Sci. 1996 Sep;21(9):351-5 PMID: 8870500
  23. hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II.
    EMBO J. 1996 Sep 16;15(18):5022-31 PMID: 8890175
  24. Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain.
    Nature. 1996 Nov 28;384(6607):375-8 PMID: 8934526
  25. Purification of a Tat-associated kinase reveals a TFIIH complex that modulates HIV-1 transcription.
    EMBO J. 1997 May 15;16(10):2836-50 PMID: 9184228
  26. CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription.
    Mol Cell Biol. 1997 Oct;17(10):6029-39 PMID: 9315662
  27. Transfer of Tat and release of TAR RNA during the activation of the human immunodeficiency virus type-1 transcription elongation complex.
    EMBO J. 1997 Sep 1;16(17):5260-72 PMID: 9311986
  28. Taking a new TAK on tat transactivation.
    Genes Dev. 1997 Oct 15;11(20):2593-9 PMID: 9334323
  29. Transcription elongation factor P-TEFb is required for HIV-1 tat transactivation in vitro.
    Genes Dev. 1997 Oct 15;11(20):2622-32 PMID: 9334325
  30. P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro.
    Genes Dev. 1997 Oct 15;11(20):2633-44 PMID: 9334326
  31. The HIV transactivator TAT binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II.
    Genes Dev. 1997 Oct 15;11(20):2645-57 PMID: 9334327
  32. A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA.
    Cell. 1998 Feb 20;92(4):451-62 PMID: 9491887
  33. Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation.
    J Mol Biol. 1998 Mar 27;277(2):179-97 PMID: 9514752
  34. PITALRE, the catalytic subunit of TAK, is required for human immunodeficiency virus Tat transactivation in vivo.
    J Virol. 1998 May;72(5):4448-53 PMID: 9557739
  35. GAL4 derivatives function alone and synergistically with mammalian activators in vitro.
    Cell. 1988 Aug 26;54(5):659-64 PMID: 3044607
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.19.2992
Published
1998-10-01
Pages
2992-6
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317190
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.12.19.2992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2