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PMID: 7835343 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Novel mechanism and factor for regulation by HIV-1 Tat.

The EMBO journal ·Vol. 14 ·No. 2 ·1995-01-16 ·Pages 321-8

Zhou Q, Sharp PA

Abstract

Tat regulation of human immunodeficiency virus (HIV) transcription is unique because of its specificity for an RNA target, TAR, and its ability to increase the efficiency of elongation by polymerase. A reconstituted reaction that is Tat-specific and TAR-dependent for activation of HIV transcription has been used to identify and partially purify a cellular activity that is required for trans-activation by Tat, but not by other activators. In the reaction, Tat stimulates the efficiency of elongation by polymerase, whereas Sp1 and other DNA sequence-specific transcription factors activate the rate of initiation. Furthermore, while TATA binding protein (TBP)-associated factors (TAFs) in the TFIID complex are required for activation by transcription factors, they are dispensable for Tat function. Thus, Tat acts through a novel mechanism, which is mediated by a specific host cellular factor, to stimulate HIV-1 gene expression.

MeSH Terms
Fungal Proteins/metabolism Gene Expression Regulation, Viral Gene Products, tat/physiology HIV-1/genetics HeLa Cells Humans Trans-Activators/metabolism Transcription Factors/metabolism Transcription, Genetic Transcriptional Activation tat Gene Products, Human Immunodeficiency Virus
Chemicals
Fungal Proteins Gal-VP16 Gene Products, tat Trans-Activators Transcription Factors tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhou Q
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Sharp P A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-01-16
Pages
321-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398086
Subset
IM
Grants
NIAID NIH HHS · AI32486 · United States
NCI NIH HHS · CA14051 · United States
NIGMS NIH HHS · GM34277 · United States
External Links
PubMed source PMC Full Text View journal details
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