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PMID: 976016 Published · ppublish English Journal Article

Sister chromatid differentiation and exchanges in adult mudminnows (Umbra limi) after in vivo exposure to 5-bromodeoxyuridine.

Chromosoma ·Vol. 56 ·No. 2 ·1976-06-30 ·Pages 101-9

Kligerman AD, Bloom SE

Abstract

An in vivo system for the detection of sister chromatid exchange (SCE) in the central mudminnow, Umbra limi, is presented. Sister chromatid differential (SCD) and SCE were demonstrated by fluorescent and Giemsa procedures 5 to 6 days after the fish were injected with 500 mug/g of BrdU. The exchange rate was found to be 2.64 SCEs metaphase in the intestines and 2.42 SCEs/metaphase in the gills. SCE analysis in U. limi should be a useful tool for measuring the mutagenicity of water-borne chemicals.

MeSH Terms
Animals Bromodeoxyuridine/pharmacology Chromatids/drug effects Crossing Over, Genetic/drug effects Drug Evaluation, Preclinical/methods Fishes Models, Biological Mutagens Water Pollutants, Chemical/adverse effects
Chemicals
Mutagens Water Pollutants, Chemical Bromodeoxyuridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kligerman A D
Bloom S E
References (24)
24 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00293110
Published
1976-06-30
Pages
101-9
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00293110 View journal details
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