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PMID: 9755175 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Srb/mediator proteins interact functionally and physically with transcriptional repressor Sfl1.

The EMBO journal ·Vol. 17 ·No. 19 ·1998-10-01 ·Pages 5757-65

Song W, Carlson M

Abstract

Srb/mediator proteins that are associated with RNA polymerase II holoenzyme have been implicated in transcriptional repression in Saccharomyces cerevisiae. We show here that the defect in repression of SUC2 caused by mutation of SRB8, SRB9, SRB11, SIN4 or ROX3 is suppressed by increased dosage of the SFL1 gene, and the genetic behavior of the sfl1Delta mutation provides further evidence for a functional relationship. Sfl1 acts on SUC2 through a repression site located immediately 5' to the TATA box, and Sfl1 binds this DNA sequence in vitro. Moreover, LexA-Sfl1 represses transcription of a reporter, and repression is reduced in an srb9 mutant. Finally, we show that Sfl1 co-immunoprecipitates from cell extracts with Srb9, Srb11, Sin4 and Rox3. We propose that Sfl1, when bound to its site, interacts with Srb/mediator proteins to inhibit transcription by RNA polymerase II holoenzyme.

MeSH Terms
Bacterial Proteins/genetics,metabolism Binding Sites Cyclin-Dependent Kinases Cyclins DNA-Binding Proteins Fungal Proteins/genetics,metabolism Gene Dosage Gene Expression Regulation, Fungal Genes, Reporter Glycoside Hydrolases/biosynthesis,genetics Mediator Complex Models, Genetic Mutation Precipitin Tests Protein Binding RNA Polymerase II/metabolism Recombinant Fusion Proteins/metabolism Repressor Proteins/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Serine Endopeptidases/genetics,metabolism TATA Box Trans-Activators Transcription Factors/genetics,metabolism Transcription, Genetic beta-Fructofuranosidase
Chemicals
Bacterial Proteins Cyclins DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae LexA protein, Bacteria Mediator Complex ROX3 protein, S cerevisiae Recombinant Fusion Proteins Repressor Proteins SFL1 protein, S cerevisiae SIN4 protein, S cerevisiae SSN2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors SSN8 protein, S cerevisiae Cyclin-Dependent Kinases RNA Polymerase II Glycoside Hydrolases beta-Fructofuranosidase Serine Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Song W
Departments of Genetics and Development, Columbia University, 701 W. 168th St., New York, NY 10032, USA.
Carlson M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.19.5757
Published
1998-10-01
Pages
5757-65
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170903
Subset
IM
Grants
NIGMS NIH HHS · GM47259 · United States
External Links
PubMed source DOI: 10.1093/emboj/17.19.5757 PMC Full Text View journal details
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