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PMID: 9742130 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of rCop-1, a new member of the CCN protein family, as a negative regulator for cell transformation.

Molecular and cellular biology ·Vol. 18 ·No. 10 ·1998-10-00 ·Pages 6131-41

Zhang R, Averboukh L, Zhu W, Zhang H, Jo H, Dempsey PJ, Coffey RJ, Pardee AB, Liang P

Abstract

By using a model system for cell transformation mediated by the cooperation of the activated H-ras oncogene and the inactivated p53 tumor suppressor gene, rCop-1 was identified by mRNA differential display as a gene whose expression became lost after cell transformation. Homology analysis indicates that rCop-1 belongs to an emerging cysteine-rich growth regulator family called CCN, which includes connective-tissue growth factor, CYR61, CEF10 (v-src inducible), and the product of the nov proto-oncogene. Unlike the other members of the CCN gene family, rCop-1 is not an immediate-early gene, it lacks the conserved C-terminal domain which was shown to confer both growth-stimulating and heparin-binding activities, and its expression is lost in cells transformed by a variety of mechanisms. Ectopic expression of rCop-1 by retroviral gene transfers led to cell death in a transformation-specific manner. These results suggest that rCop-1 represents a new class of CCN family proteins that have functions opposing those of the previously identified members.

MeSH Terms
Aging Amino Acid Sequence Animals Apoptosis Base Sequence CCN Intercellular Signaling Proteins Carcinogenicity Tests Cell Cycle Cell Line Cell Transformation, Neoplastic Fibroblasts/metabolism Gene Expression Genes, Viral Genes, p53 Genes, ras Mice Molecular Sequence Data Rats Repressor Proteins/genetics,metabolism Retroviridae/genetics Rodentia/embryology Sequence Homology, Amino Acid Subcellular Fractions
Chemicals
CCN Intercellular Signaling Proteins CCN5 protein, rat Repressor Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Zhang R
Vanderbilt Cancer Center, Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232, USA.
Averboukh L
Zhu W
Zhang H
Jo H
Dempsey P J
Coffey R J
Pardee A B
Liang P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.10.6131
Published
1998-10-00
Pages
6131-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC109199
Subset
IM
Grants
NCI NIH HHS · CA68485 · United States
NCI NIH HHS · CA74067 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.10.6131 PMC Full Text View journal details
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