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PMID: 9740129 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Distinct functions of the closely related tandem RNA-recognition motifs of hnRNP A1.

RNA (New York, N.Y.) ·Vol. 4 ·No. 9 ·1998-09-00 ·Pages 1111-23

Mayeda A, Munroe SH, Xu RM, Krainer AR

Abstract

hnRNP A1 regulates alternative splicing by antagonizing SR proteins. It consists of two closely related, tandem RNA-recognition motifs (RRMs), followed by a glycine-rich domain. Analysis of variant proteins with duplications, deletions, or swaps of the RRMs showed that although both RRMs are required for alternative splicing function, each RRM plays distinct roles, and their relative position is important. Surprisingly, RRM2 but not RRM1 could support this function when duplicated, despite their very similar structure. Specific RNA binding and annealing are not sufficient for hnRNP A1 alternative splicing function. These observations, together with phylogenetic and structural data, suggest that the two RRMs are quasi-symmetric but functionally nonequivalent modules that evolved as components of a single bipartite domain.

MeSH Terms
Alternative Splicing Amino Acid Sequence Animals Base Sequence Binding Sites/genetics Conserved Sequence Escherichia coli/genetics Evolution, Molecular Genetic Variation Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Humans Molecular Sequence Data Phylogeny RNA, Heterogeneous Nuclear/chemistry,genetics,metabolism Recombinant Proteins/chemistry,genetics,metabolism Ribonucleoproteins/chemistry,genetics,metabolism Sequence Homology, Amino Acid
Chemicals
Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins RNA, Heterogeneous Nuclear Recombinant Proteins Ribonucleoproteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mayeda A
Cold Spring Harbor Laboratory, New York 11724-2208, USA.
Munroe S H
Xu R M
Krainer A R
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s135583829898089x
Published
1998-09-00
Pages
1111-23
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369686
Subset
IM
Grants
NCI NIH HHS · CA13106 · United States
NIDDK NIH HHS · DK40834 · United States
NIGMS NIH HHS · GM55874 · United States
External Links
PubMed source DOI: 10.1017/s135583829898089x PMC Full Text View journal details
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