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PMID: 9724639 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.

The EMBO journal ·Vol. 17 ·No. 17 ·1998-09-01 ·Pages 5037-47

Palmer A, Gavin AC, Nebreda AR

Abstract

M-phase entry in eukaryotic cells is driven by activation of MPF, a regulatory factor composed of cyclin B and the protein kinase p34(cdc2). In G2-arrested Xenopus oocytes, there is a stock of p34(cdc2)/cyclin B complexes (pre-MPF) which is maintained in an inactive state by p34(cdc2) phosphorylation on Thr14 and Tyr15. This suggests an important role for the p34(cdc2) inhibitory kinase(s) such as Wee1 and Myt1 in regulating the G2-->M transition during oocyte maturation. MAP kinase (MAPK) activation is required for M-phase entry in Xenopus oocytes, but its precise contribution to the activation of pre-MPF is unknown. Here we show that the C-terminal regulatory domain of Myt1 specifically binds to p90(rsk), a protein kinase that can be phosphorylated and activated by MAPK. p90(rsk) in turn phosphorylates the C-terminus of Myt1 and down-regulates its inhibitory activity on p34(cdc2)/cyclin B in vitro. Consistent with these results, Myt1 becomes phosphorylated during oocyte maturation, and activation of the MAPK-p90(rsk) cascade can trigger some Myt1 phosphorylation prior to pre-MPF activation. We found that Myt1 preferentially associates with hyperphosphorylated p90(rsk), and complexes can be detected in immunoprecipitates from mature oocytes. Our results suggest that during oocyte maturation MAPK activates p90(rsk) and that p90(rsk) in turn down-regulates Myt1, leading to the activation of p34(cdc2)/cyclin B.

MeSH Terms
Animals CDC2 Protein Kinase/metabolism Calcium-Calmodulin-Dependent Protein Kinases/metabolism Cell Differentiation Cyclin B/metabolism Enzyme Activation Models, Biological Oocytes/cytology,metabolism Phosphorylation Protein Binding Protein Serine-Threonine Kinases/antagonists & inhibitors Protein-Tyrosine Kinases/antagonists & inhibitors Ribosomal Protein S6 Kinases/metabolism Signal Transduction Xenopus Xenopus Proteins
Chemicals
Cyclin B Xenopus Proteins MYT1 kinase, Xenopus Protein-Tyrosine Kinases Protein Serine-Threonine Kinases Ribosomal Protein S6 Kinases Calcium-Calmodulin-Dependent Protein Kinases CDC2 Protein Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Palmer A
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Gavin A C
Nebreda A R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.17.5037
Published
1998-09-01
Pages
5037-47
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170831
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1093/emboj/17.17.5037 PMC Full Text View journal details
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