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PMID: 9716404 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast.

Genes & development ·Vol. 12 ·No. 16 ·1998-08-15 ·Pages 2510-21

McNeil JB, Agah H, Bentley D

Abstract

We investigated whether the multisubunit holoenzyme complex of RNA polymerase II (Pol II) and mediator is universally required for transcription in budding yeast. DeltaCTD Pol II lacking the carboxy-terminal domain of the large subunit cannot assemble with mediator but can still transcribe the CUP1 gene. CUP1 transcripts made by DeltaCTD Pol II initiated correctly and some extended past the normal poly(A) site yielding a novel dicistronic mRNA. Most CUP1 transcripts made by DeltaCTD Pol II were degraded but could be stabilized by deletion of the XRN1 gene. Unlike other genes, transcription of CUP1 and HSP82 also persisted after inactivation of the CTD kinase Kin28 or the mediator subunit Srb4. The upstream-activating sequence (UAS) of the CUP1 promoter was sufficient to drive Cu2+ inducible transcription without Srb4 and heat shock inducible transcription without the CTD. We conclude that the Pol II holoenzyme is not essential for all UAS-dependent activated transcription in yeast.

MeSH Terms
Carrier Proteins Coenzymes/metabolism Cyclin-Dependent Kinases Fungal Proteins/physiology Gene Expression Regulation, Fungal Mediator Complex Metallothionein/genetics,metabolism Promoter Regions, Genetic Protein Serine-Threonine Kinases/physiology RNA Polymerase II/metabolism RNA, Messenger/biosynthesis Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Transcription Factors/physiology Transcriptional Activation
Chemicals
CUP1-1 protein, S cerevisiae Carrier Proteins Coenzymes Fungal Proteins Mediator Complex RNA, Messenger SRB4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors copper thionein Metallothionein Protein Serine-Threonine Kinases Cyclin-Dependent Kinases Kin28 protein kinase, S cerevisiae RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McNeil J B
Amgen Institute, Ontario Cancer Institute, Toronto, Ontario M5G 2C1, Canada.
Agah H
Bentley D
References (57)
57 references, click to expand
  1. RNA polymerase II C-terminal repeat influences response to transcriptional enhancer signals.
    Nature. 1990 Oct 4;347(6292):491-4 PMID: 2215664
  2. Transcriptional activation independent of TFIIH kinase and the RNA polymerase II mediator in vivo.
    Nature. 1998 May 28;393(6683):389-92 PMID: 9620805
  3. Role of heat shock transcription factor in yeast metallothionein gene expression.
    Mol Cell Biol. 1991 Jul;11(7):3676-81 PMID: 1904545
  4. RNA polymerase II carboxy-terminal domain contributes to the response to multiple acidic activators in vitro.
    Genes Dev. 1991 Dec;5(12B):2431-40 PMID: 1752437
  5. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors.
    Yeast. 1991 Aug-Sep;7(6):609-15 PMID: 1767589
  6. Nucleosome loss activates CUP1 and HIS3 promoters to fully induced levels in the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Apr;12(4):1621-9 PMID: 1549116
  7. Tandemly duplicated upstream control sequences mediate copper-induced transcription of the Saccharomyces cerevisiae copper-metallothionein gene.
    Mol Cell Biol. 1986 Apr;6(4):1158-63 PMID: 3537699
  8. An RNA polymerase I enhancer in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jun;6(6):2089-97 PMID: 3537713
  9. Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.
    Mol Cell Biol. 1987 May;7(5):1602-11 PMID: 3299050
  10. Functional redundancy and structural polymorphism in the large subunit of RNA polymerase II.
    Cell. 1987 Sep 11;50(6):909-15 PMID: 3304659
  11. Mutations in RNA polymerase II enhance or suppress mutations in GAL4.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2794-8 PMID: 2495535
  12. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  13. Transcription of the 5 S rRNA gene of Saccharomyces cerevisiae requires a promoter element at +1 and a 14-base pair internal control region.
    J Biol Chem. 1989 Nov 25;264(33):20060-7 PMID: 2684967
  14. Intragenic and extragenic suppressors of mutations in the heptapeptide repeat domain of Saccharomyces cerevisiae RNA polymerase II.
    Genetics. 1989 Dec;123(4):715-24 PMID: 2693207
  15. RNA polymerase II subunit composition, stoichiometry, and phosphorylation.
    Mol Cell Biol. 1990 May;10(5):1915-20 PMID: 2183013
  16. The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in vitro.
    J Biol Chem. 1990 May 25;265(15):8351-3 PMID: 2187861
  17. Transcription initiation complexes and upstream activation with RNA polymerase II lacking the C-terminal domain of the largest subunit.
    Mol Cell Biol. 1990 Oct;10(10):5562-4 PMID: 2398901
  18. The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II.
    Mol Cell Biol. 1992 May;12(5):2250-9 PMID: 1569952
  19. Defects in mRNA 3'-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure.
    Genes Dev. 1992 Oct;6(10):1914-26 PMID: 1398069
  20. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast.
    Cell. 1993 Jul 2;73(7):1361-75 PMID: 8324825
  21. Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure.
    Mol Cell Biol. 1993 Aug;13(8):4826-35 PMID: 8336719
  22. RNA polymerases IIA and IIO have distinct roles during transcription from the TATA-less murine dihydrofolate reductase promoter.
    J Biol Chem. 1993 Nov 25;268(33):25033-40 PMID: 8227067
  23. The kin28 protein kinase is associated with a cyclin in Saccharomyces cerevisiae.
    J Mol Biol. 1993 Nov 20;234(2):307-10 PMID: 8230216
  24. An RNA polymerase II holoenzyme responsive to activators.
    Nature. 1994 Mar 31;368(6470):466-9 PMID: 8133894
  25. Interplay of positive and negative effectors in function of the C-terminal repeat domain of RNA polymerase II.
    Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2362-6 PMID: 8134400
  26. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
    Cell. 1994 May 20;77(4):599-608 PMID: 8187178
  27. Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation.
    Nature. 1994 Jul 7;370(6484):75-7 PMID: 8015613
  28. RNA polymerase II C-terminal domain required for enhancer-driven transcription.
    Nature. 1995 Apr 13;374(6523):660-2 PMID: 7715709
  29. General requirement for RNA polymerase II holoenzymes in vivo.
    Proc Natl Acad Sci U S A. 1995 May 9;92(10):4587-90 PMID: 7753848
  30. KIN28 encodes a C-terminal domain kinase that controls mRNA transcription in Saccharomyces cerevisiae but lacks cyclin-dependent kinase-activating kinase (CAK) activity.
    Mol Cell Biol. 1995 Jun;15(6):2983-92 PMID: 7760796
  31. Association of an activator with an RNA polymerase II holoenzyme.
    Genes Dev. 1995 Apr 15;9(8):897-910 PMID: 7774808
  32. The KIN28 gene is required both for RNA polymerase II mediated transcription and phosphorylation of the Rpb1p CTD.
    J Mol Biol. 1995 Jun 9;249(3):535-44 PMID: 7783209
  33. The TBP-TFIIA interaction in the response to acidic activators in vivo.
    Science. 1995 Jul 7;269(5220):75-8 PMID: 7604282
  34. A mammalian RNA polymerase II holoenzyme containing all components required for promoter-specific transcription initiation.
    Cell. 1995 Oct 6;83(1):137-46 PMID: 7553866
  35. Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations.
    Genetics. 1995 Aug;140(4):1223-33 PMID: 7498765
  36. RNA polymerase II holoenzyme contains SWI/SNF regulators involved in chromatin remodeling.
    Cell. 1996 Jan 26;84(2):235-44 PMID: 8565069
  37. A human RNA polymerase II complex associated with SRB and DNA-repair proteins.
    Nature. 1996 May 2;381(6577):86-9 PMID: 8609996
  38. Reversible phosphorylation of the C-terminal domain of RNA polymerase II.
    J Biol Chem. 1996 Aug 9;271(32):19009-12 PMID: 8759772
  39. Transcription activation in cells lacking TAFIIS.
    Nature. 1996 Sep 12;383(6596):185-8 PMID: 8774886
  40. TBP-associated factors are not generally required for transcriptional activation in yeast.
    Nature. 1996 Sep 12;383(6596):188-91 PMID: 8774887
  41. Gene activation by recruitment of the RNA polymerase II holoenzyme.
    Genes Dev. 1996 Sep 15;10(18):2359-67 PMID: 8824594
  42. Distinct activated and non-activated RNA polymerase II complexes in yeast.
    EMBO J. 1996 Sep 2;15(17):4654-64 PMID: 8887556
  43. Essential yeast protein with unexpected similarity to subunits of mammalian cleavage and polyadenylation specificity factor (CPSF).
    Science. 1996 Nov 29;274(5292):1511-4 PMID: 8929408
  44. Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeast.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):337-41 PMID: 6364141
  45. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription.
    Nature. 1997 Jan 23;385(6614):357-61 PMID: 9002523
  46. Cdc73p and Paf1p are found in a novel RNA polymerase II-containing complex distinct from the Srbp-containing holoenzyme.
    Mol Cell Biol. 1997 Mar;17(3):1160-9 PMID: 9032243
  47. Transcriptional activation by recruitment.
    Nature. 1997 Apr 10;386(6625):569-77 PMID: 9121580
  48. Modulation of RNA polymerase II elongation efficiency by C-terminal heptapeptide repeat domain kinase I.
    J Biol Chem. 1997 Apr 25;272(17):10990-3 PMID: 9110987
  49. Evidence for a mediator cycle at the initiation of transcription.
    Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6075-8 PMID: 9177171
  50. Yeast TAF(II)145 functions as a core promoter selectivity factor, not a general coactivator.
    Cell. 1997 Aug 22;90(4):615-24 PMID: 9288742
  51. Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators.
    J Biol Chem. 1997 Sep 26;272(39):24563-71 PMID: 9305922
  52. 5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II.
    Genes Dev. 1997 Dec 15;11(24):3306-18 PMID: 9407024
  53. mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain.
    Genes Dev. 1997 Dec 15;11(24):3319-26 PMID: 9407025
  54. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain.
    Genes Dev. 1998 Jan 1;12(1):45-54 PMID: 9420330
  55. Genetics of transcriptional regulation in yeast: connections to the RNA polymerase II CTD.
    Annu Rev Cell Dev Biol. 1997;13:1-23 PMID: 9442866
  56. RNA polymerase I-promoted HIS4 expression yields uncapped, polyadenylated mRNA that is unstable and inefficiently translated in Saccharomyces cerevisiae.
    Mol Cell Biol. 1998 Feb;18(2):665-75 PMID: 9447962
  57. Heat shock transcription factor activates transcription of the yeast metallothionein gene.
    Mol Cell Biol. 1991 Mar;11(3):1232-8 PMID: 1996089
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.16.2510
Published
1998-08-15
Pages
2510-21
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317099
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.12.16.2510 PMC Full Text View journal details
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