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PMID: 8887556 Published · ppublish English Journal Article

Distinct activated and non-activated RNA polymerase II complexes in yeast.

The EMBO journal ·Vol. 15 ·No. 17 ·1996-09-02 ·Pages 4654-64

Akhtar A, Faye G, Bentley DL

Abstract

We used a transcriptional run-on assay in permeabilized yeast cells to study the distribution of RNA polymerase II (pol II) complexes before and after activation by Gal4. Polymerases were found engaged on the gene at the 5' end before activation, but only appeared at the 3' end after activation. Mutations of the pol II C-terminal domain (CTD), the CTD kinase Kin28 and the holoenzyme subunit Srb2 all inhibited the formation of 3' polymerases in response to activator. However, these mutations did not inhibit the establishment of polymerases at the 5' end. The differences between 3' and 5' ternary complexes suggest that they represent qualitatively distinct 'activated' and 'non-activated' forms of polymerase. The results implicate CTD phosphorylation in a switch from 'non-activated' transcription, which is confined to the 5' end, to an 'activated' mode that traverses the length of the gene.

MeSH Terms
DNA-Binding Proteins Enzyme Activation Fungal Proteins/metabolism Mutation RNA Polymerase II/genetics,metabolism Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic
Chemicals
DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Akhtar A
Molecular Genetics of Differentiation Laboratory, Imperial Cancer Research Fund, London, UK.
Faye G
Bentley D L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-09-02
Pages
4654-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452197
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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