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PMID: 9707603 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A peptidoglycan recognition protein in innate immunity conserved from insects to humans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 17 ·1998-08-18 ·Pages 10078-82

Kang D, Liu G, Lundström A, Gelius E, Steiner H

Abstract

Innate nonself recognition must rely on common structures of invading microbes. In a differential display screen for up-regulated immune genes in the moth Trichoplusia ni we have found mechanisms for recognition of bacterial cell wall fragments. One bacteria-induced gene encodes a protein that, after expression in the baculovirus system, was shown to be a peptidoglycan recognition protein (PGRP). It binds strongly to Gram-positive bacteria. We have also cloned the corresponding cDNA from mouse and human and shown this gene to be expressed in a variety of organs, notably organs of the immune system-i.e., bone marrow and spleen. In addition, purified recombinant murine PGRP was shown to possess peptidoglycan affinity. From our results and the sequence homology, we conclude that PGRP is a ubiquitous protein involved in innate immunity, conserved from insects to humans.

MeSH Terms
Amino Acid Sequence Animals Bacteria/immunology Baculoviridae/genetics Base Sequence Conserved Sequence Humans Immunity/genetics Insect Proteins/genetics,immunology,metabolism Male Mice Molecular Sequence Data Moths/genetics,immunology Peptidoglycan/immunology,metabolism Polymerase Chain Reaction Protein Binding Proteins/genetics,immunology,metabolism RNA, Messenger/genetics,metabolism Recombinant Proteins/genetics,immunology Sequence Homology, Amino Acid Tissue Distribution
Chemicals
Insect Proteins Peptidoglycan Proteins RNA, Messenger Recombinant Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kang D
Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden.
Liu G
Lundström A
Gelius E
Steiner H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.17.10078
Published
1998-08-18
Pages
10078-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21464
Subset
IM
Databases
GENBANK
AF076481, AF076482, AF076483
External Links
PubMed source DOI: 10.1073/pnas.95.17.10078 PMC Full Text View journal details
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