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PMID: 9693142 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm.

Development (Cambridge, England) ·Vol. 125 ·No. 17 ·1998-09-00 ·Pages 3389-97

Yamamoto A, Amacher SL, Kim SH, Geissert D, Kimmel CB, De Robertis EM

Abstract

Zebrafish paraxial protocadherin (papc) encodes a transmembrane cell adhesion molecule (PAPC) expressed in trunk mesoderm undergoing morphogenesis. Microinjection studies with a dominant-negative secreted construct suggest that papc is required for proper dorsal convergence movements during gastrulation. Genetic studies show that papc is a close downstream target of spadetail, gene encoding a transcription factor required for mesodermal morphogenetic movements. Further, we show that the floating head homeobox gene is required in axial mesoderm to repress the expression of both spadetail and papc, promoting notochord and blocking differentiation of paraxial mesoderm. The PAPC structural cell-surface protein may provide a link between regulatory transcription factors and the actual cell biological behaviors that execute morphogenesis during gastrulation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Body Patterning/genetics Cadherins/genetics,physiology Cell Differentiation/genetics Cloning, Molecular DNA Primers/genetics Gastrula/cytology Gene Expression Regulation, Developmental Genes, Homeobox In Situ Hybridization Mesoderm/cytology Models, Genetic Molecular Sequence Data Morphogenesis/genetics Polymerase Chain Reaction Sequence Homology, Amino Acid Somites/cytology Transcription Factors/genetics,physiology Zebrafish/embryology,genetics,physiology
Chemicals
Cadherins DNA Primers Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yamamoto A
Howard Hughes Medical Institute, Department of Biological Chemistry, University of California, Los Angeles, California 90095-1662, USA.
Amacher S L
Kim S H
Geissert D
Kimmel C B
De Robertis E M
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Article Info
Journal
Development (Cambridge, England)
Abbr.
Development
ISSN
0950-1991
Published
1998-09-00
Pages
3389-97
Language
English
Region
England
NLM ID
8701744
PMCID
PMC2280034
Subset
IM
Grants
NICHD NIH HHS · R37 HD021502-13 · United States
NICHD NIH HHS · HD22486 · United States
NICHD NIH HHS · R37 HD021502 · United States
NICHD NIH HHS · HD51202 · United States
NICHD NIH HHS · P01 HD022486 · United States
NICHD NIH HHS · R01 HD021502 · United States
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PubMed source PMC Full Text View journal details
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