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PMID: 9393857 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Avian hairy gene expression identifies a molecular clock linked to vertebrate segmentation and somitogenesis.

Cell ·Vol. 91 ·No. 5 ·1997-11-28 ·Pages 639-48

Palmeirim I, Henrique D, Ish-Horowicz D, Pourquié O

Abstract

We have identified and characterized c-hairy1, an avian homolog of the Drosophila segmentation gene, hairy. c-hairy1 is strongly expressed in the presomitic mesoderm, where its mRNA exhibits cyclic waves of expression whose temporal periodicity corresponds to the formation time of one somite (90 min). The apparent movement of these waves is due to coordinated pulses of c-hairy1 expression, not to cell displacement along the anteroposterior axis, nor to propagation of an activating signal. Rather, the rhythmic c-hairy mRNA expression is an autonomous property of the paraxial mesoderm. These results provide molecular evidence for a developmental clock linked to segmentation and somitogenesis of the paraxial mesoderm, and support the possibility that segmentation mechanisms used by invertebrates and vertebrates have been conserved.

MeSH Terms

Animals Avian Proteins Basic Helix-Loop-Helix Transcription Factors Biological Clocks/genetics Cell Movement/genetics Chick Embryo Cycloheximide Drosophila/genetics Gene Expression Regulation, Developmental/physiology Mesoderm/cytology,physiology Molecular Sequence Data Periodicity Protein Synthesis Inhibitors Proteins/genetics RNA, Messenger/analysis Sequence Homology, Amino Acid Somites/cytology,physiology Vertebrates

Chemicals

Avian Proteins Basic Helix-Loop-Helix Transcription Factors Protein Synthesis Inhibitors Proteins RNA, Messenger hairy1 protein, Gallus gallus Cycloheximide

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Palmeirim I

Institut d'Embryologie Cellulaire et Moléculaire du Centre National de la Recherche Scientifique et du Collège de France, Nogent sur Marne.

Henrique D

Ish-Horowicz D

Pourquié O

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(00)80451-1

Published

1997-11-28

Pages

639-48

Language

English

Region

United States

NLM ID

0413066

Subset

Databases

GENBANK

AF032966, D13418, D14029, D16464, L04527, L19314, S29712, S48025, U36194, X15904, X16550, X16552, X16553, X95301, X97329, X97330, X97331, X97332, X97333

SWISSPROT

Q03062

External Links

PubMed source DOI: 10.1016/s0092-8674(00)80451-1 View journal details

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Avian hairy gene expression identifies a molecular clock linked to vertebrate segmentation and somitogenesis.

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