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PMID: 9689056 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Unusual proliferation arrest and transcriptional control properties of a newly discovered E2F family member, E2F-6.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 16 ·1998-08-04 ·Pages 9190-5

Gaubatz S, Wood JG, Livingston DM

Abstract

E2F transcription factors play an important role in the regulation of cell cycle progression. We report here the cloning and characterization of an additional member of this family, E2F-6. E2F-6 lacks pocket protein binding and transactivation domains, and it is a potent transcriptional repressor that contains a modular repression domain at its carboxyl terminus. Overproduction of E2F-6 had no specific effect on cell cycle progression in asynchronously growing Saos2 and NIH 3T3 cells, but it inhibited entry into S phase of NIH 3T3 cells stimulated to exit G0. Taken together, these data suggest that E2F-6 can regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Base Sequence Binding Sites Cloning, Molecular Consensus Sequence DNA, Complementary E2F6 Transcription Factor HeLa Cells Humans Mice Molecular Sequence Data Recombinant Proteins/genetics,metabolism Repressor Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
DNA, Complementary E2F6 Transcription Factor E2F6 protein, human E2f6 protein, mouse Recombinant Proteins Repressor Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gaubatz S
Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA.
Wood J G
Livingston D M
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.16.9190
Published
1998-08-04
Pages
9190-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21314
Subset
IM
Databases
GENBANK
AF059292
External Links
PubMed source DOI: 10.1073/pnas.95.16.9190 PMC Full Text View journal details
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