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PMID: 9501179 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

E2F-6, a member of the E2F family that can behave as a transcriptional repressor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 6 ·1998-03-17 ·Pages 2850-5

Trimarchi JM, Fairchild B, Verona R, Moberg K, Andon N, Lees JA

Abstract

The E2F family of proteins is required to establish the correct cell-cycle-dependent transcription of genes that direct the process of cell division. All previously identified E2F proteins can act in a similar manner; depending on whether or not they are associated with the cell cycle inhibitors the retinoblastoma protein (pRB), p107, or p130, they can either repress or activate the transcription of E2F-responsive genes. We now report the cloning and characterization of another E2F family member, E2F-6, whose structure is reminiscent of the dominant inhibitors of other transcription factor families. The dimerization and DNA binding properties of E2F-6 are similar to those of the other E2F family members. However, it is not regulated by pRB, p107, or p130, and it is unable to activate transcription. Instead, it can act to repress the transcription of E2F responsive genes by countering the activity of the other E2F complexes via a pRB-, p107-, or p130-independent mechanism.

MeSH Terms
Amino Acid Sequence Animals Antibody Specificity Cloning, Molecular DNA, Complementary/genetics Dimerization E2F6 Transcription Factor Gene Expression Regulation Genes, Reporter Humans Mice Molecular Sequence Data Multigene Family Protein Binding Repressor Proteins/genetics Retinoblastoma Protein/metabolism Sequence Homology, Amino Acid Tissue Distribution Transcription Factors/genetics,immunology,metabolism
Chemicals
DNA, Complementary E2F6 Transcription Factor E2F6 protein, human E2f6 protein, mouse Repressor Proteins Retinoblastoma Protein Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Trimarchi J M
Center for Cancer Research, Massachusetts Institute of Technology, Building E17-517B, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Fairchild B
Verona R
Moberg K
Andon N
Lees J A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.6.2850
Published
1998-03-17
Pages
2850-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19658
Subset
IM
Databases
GENBANK
AF041381
External Links
PubMed source DOI: 10.1073/pnas.95.6.2850 PMC Full Text View journal details
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