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PMID: 9649434 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overlapping functions of components of a bacterial Sec-independent protein export pathway.

The EMBO journal ·Vol. 17 ·No. 13 ·1998-07-01 ·Pages 3640-50

Sargent F, Bogsch EG, Stanley NR, Wexler M, Robinson C, Berks BC, Palmer T

Abstract

We describe the identification of two Escherichia coli genes required for the export of cofactor-containing periplasmic proteins, synthesized with signal peptides containing a twin arginine motif. Both gene products are homologous to the maize HCF106 protein required for the translocation of a subset of lumenal proteins across the thylakoid membrane. Disruption of either gene affects the export of a range of such proteins, and a complete block is observed when both genes are inactivated. The Sec protein export pathway was unaffected, indicating the involvement of the gene products in a novel export system. The accumulation of active cofactor-containing proteins in the cytoplasm of the mutant strains suggests a role for the gene products in the translocation of folded proteins. One of the two HCF106 homologues is encoded by the first gene of a four cistron operon, tatABCD, and the second by an unlinked gene, tatE. A mutation previously assigned to the hcf106 homologue encoded at the tatABCD locus, mttA, lies instead in the tatB gene.

MeSH Terms
Bacterial Proteins/genetics,physiology Base Sequence Biological Transport Cloning, Molecular Cytoplasm/metabolism DNA, Bacterial Enzyme Activation Escherichia coli/genetics,growth & development,physiology Formate Dehydrogenases/metabolism Genes, Bacterial Hydrogenase/metabolism Membrane Proteins/chemistry Molecular Sequence Data Mutagenesis Oxidoreductases, N-Demethylating/genetics,metabolism Phenotype Plant Proteins Protein Precursors/metabolism Protein Processing, Post-Translational Recombinant Fusion Proteins/metabolism
Chemicals
Bacterial Proteins DNA, Bacterial Hcf106 protein, plant Membrane Proteins Plant Proteins Protein Precursors Recombinant Fusion Proteins Hydrogenase Formate Dehydrogenases Oxidoreductases, N-Demethylating trimethylamine dehydrogenase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sargent F
Nitrogen Fixation Laboratory, John Innes Centre, Colney, Norwich NR4 7UH.
Bogsch E G
Stanley N R
Wexler M
Robinson C
Berks B C
Palmer T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.13.3640
Published
1998-07-01
Pages
3640-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170700
Subset
IM
Databases
GENBANK
AJ005830
External Links
PubMed source DOI: 10.1093/emboj/17.13.3640 PMC Full Text View journal details
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