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PMID: 8071220 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Anaerobic regulation of the hydrogenase 1 (hya) operon of Escherichia coli.

Journal of bacteriology ·Vol. 176 ·No. 17 ·1994-09-00 ·Pages 5423-8

Brøndsted L, Atlung T

Abstract

Using a transcriptional fusion to the lacZ gene, we have analyzed the anaerobic regulation of the hydrogenase 1 (hya) operon in response to different anaerobic growth conditions and to mutations in regulatory genes. We found that the transcription of the hya operon was induced when the growth condition was changed from aerobic to anaerobic and that this induction was independent of Fnr but dependent on regulators AppY and ArcA. Furthermore, we found that the transcription of the hya operon was not regulated by the cyclic AMP-cyclic AMP receptor protein complex. Investigation of the effects of different anaerobic growth conditions on the expression of the hya operon showed that expression was induced by formate and repressed by nitrate. Formate induction was not mediated by the fhlA gene product, and nitrate repression was not mediated by the narL gene product. We found a high level of anaerobic expression of the hya operon in glucose medium supplemented with formate and in glycerol medium supplemented with fumarate, suggesting that hydrogenase isoenzyme 1 has a function during both fermentative growth and anaerobic respiration.

Related Genes
hya lacZ
MeSH Terms
Amino Acid Sequence Anaerobiosis Base Sequence Cloning, Molecular Escherichia coli/enzymology,genetics,growth & development Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes, Bacterial Genotype Hydrogenase/biosynthesis,genetics Kinetics Molecular Sequence Data Operon Plasmids Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis,metabolism Transcription, Genetic beta-Galactosidase/biosynthesis,metabolism
Chemicals
Recombinant Fusion Proteins Hydrogenase beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brøndsted L
Department of Microbiology, Technical University of Denmark, Lyngby.
Atlung T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.17.5423-5428.1994
Published
1994-09-00
Pages
5423-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196730
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.17.5423-5428.1994 PMC Full Text View journal details
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