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PMID: 6387391 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of the trimethylamine N-oxide (TMAO) reductase in Escherichia coli: analysis of tor::Mud1 operon fusion.

Molecular & general genetics : MGG ·Vol. 195 ·No. 1-2 ·1984-00-00 ·Pages 351-5

Pascal MC, Burini JF, Chippaux M

Abstract

Mud1 insertion mutants of Escherichia coli were obtained in which the lac structural genes were fused to the promoter of torA, a gene encoding the trimethylamine N-oxide (TMAO) reductase. Expression of the fusion is induced by TMAO and repressed by oxygen. However, in contrast to the nar operon which codes for the nitrate reductase structural genes, the tor::Mud1 fusion was found to be independent of the positive control exerted by the nirR gene product and not repressed by the molybdenum cofactor. The torA gene which is strongly linked to pyrF (28.3U) is different from any tor gene already described in E. coli or in Salmonella typhimurium.

MeSH Terms

Crosses, Genetic Escherichia coli/enzymology,genetics Genes Genes, Bacterial Genotype Mutation NADH, NADPH Oxidoreductases/genetics Operon Oxidoreductases Acting on CH-NH Group Donors Transcription, Genetic Transduction, Genetic

Chemicals

methylamine dehydrogenase Oxidoreductases Acting on CH-NH Group Donors NADH, NADPH Oxidoreductases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pascal M C

Burini J F

Chippaux M

References (13)

13 references, click to expand

Nitrate reductase and cytochrome bnitrate reductase structural genes as parts of the nitrate reductase operon.
Mol Gen Genet. 1981;181(4):535-40 PMID: 7022129
Roles for menaquinone and the two trimethylamine oxide (TMAO) reductases in TMAO respiration in Salmonella typhimurium: Mu d(Apr lac) insertion mutations in men and tor.
J Bacteriol. 1983 Sep;155(3):1147-55 PMID: 6309742
The chromosomal location and pleiotropic effects of mutations of the nirA+ gene of Escherichia coli K12: the essential role of nirA+ in nitrite reduction and in other anaerobic redox reactions.
J Gen Microbiol. 1978 May;106(1):1-12 PMID: 206651
Operon fusions in the nitrate reductase operon and study of the control gene nir R in Escherichia coli.
Mol Gen Genet. 1981;182(3):477-9 PMID: 6457962
Homology between CAP and Fnr, a regulator of anaerobic respiration in Escherichia coli.
J Mol Biol. 1983 May 15;166(2):241-7 PMID: 6343617
Purification and some properties of inducible tertiary amine N-oxide reductase from Escherichia coli.
J Biochem. 1979 Dec;86(6):1709-17 PMID: 393699
Isolation from Salmonella typhimurium LT2 of mutants lacking specifically nitrate reductase activity and mapping of the chl-C gene.
Mol Gen Genet. 1973 Aug 17;124(3):247-51 PMID: 4584943
Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor.
J Gen Microbiol. 1976 Dec;97(2):145-60 PMID: 796407
Hfr formation directed by tn10.
Genetics. 1979 Apr;91(4):639-55 PMID: 17248903
Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.
J Bacteriol. 1982 Aug;151(2):788-99 PMID: 7047497
Regulation of the nitrate reductase operon: effect of mutations in chlA, B, D and E genes.
Mol Gen Genet. 1982;188(1):103-6 PMID: 6757667
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3 PMID: 159458
Proton translocation coupled to trimethylamine N-oxide reduction in anaerobically grown Escherichia coli.
J Bacteriol. 1981 Dec;148(3):762-8 PMID: 7031034

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00332770

Published

1984-00-00

Pages

351-5

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00332770

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Regulation of the trimethylamine N-oxide (TMAO) reductase in Escherichia coli: analysis of tor::Mud1 operon fusion.

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