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PMID: 96437 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Pre-proparathyroid hormone; amino acid sequence, chemical synthesis, and some biological studies of the precursor region.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 6 ·1978-06-00 ·Pages 2616-20

Habener JF, Rosenblatt M, Kemper B, Kronenberg HM, Rich A, Potts JT

Abstract

The precursor of bovine proparathyroid hormone was synthesized by translation of parathyroid mRNA in a wheat-germ cell-free system. The amino acid sequence of the NH2-terminal extension (the pre sequence) was determined by repetitive Edman degradation of the polypeptide labeled with radioactive amino acids (radiosequencing). The pre sequence of pre-proparathyroid hormone is (formula: see text) which is followed by the sequence of proparathyroid hormone. It is significant that 20 of the 25 amino acids in the sequence are hydrophobic. This high hydrophobicity is consistent with the proposed role of the pre sequence as a membrane-penetrating peptide. The precursor-specific sequence of 31 amino acids was snythesized chemically by the solid-phase technique. This synthetic peptide was shown to bind to the microsomal fraction of homogenates prepared from extracts of parathyroid glands, a finding consistent with the proposed role of the precursor peptide in the attachment of the nascent chain--mRNA--ribosome complex to the endoplasmic reticulum.

MeSH Terms
Amino Acid Sequence Animals Cattle Edetic Acid/pharmacology Microsomes/metabolism Parathyroid Glands/metabolism,ultrastructure Parathyroid Hormone/metabolism Protein Binding/drug effects Protein Precursors/metabolism Structure-Activity Relationship
Chemicals
Parathyroid Hormone Protein Precursors Edetic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Habener J F
Rosenblatt M
Kemper B
Kronenberg H M
Rich A
Potts J T
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.6.2616
Published
1978-06-00
Pages
2616-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392613
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.6.2616 PMC Full Text View journal details
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