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PMID: 9596711 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Pseudomonas aeruginosa lasR transcription correlates with the transcription of lasA, lasB, and toxA in chronic lung infections associated with cystic fibrosis.

Infection and immunity ·Vol. 66 ·No. 6 ·1998-06-00 ·Pages 2521-8

Storey DG, Ujack EE, Rabin HR, Mitchell I

Abstract

The role of Pseudomonas aeruginosa quorum-sensing systems in the lung infections associated with cystic fibrosis (CF) has not been examined. The purpose of this study was to determine if genes regulated by the LasR-LasI quorum-sensing system were coordinately regulated by the P. aeruginosa populations during the lung infections associated with CF. We also wanted to ascertain if there was a relationship between the expression of lasR, a transcriptional regulator, and some P. aeruginosa virulence factors during these infections. We extracted RNAs from the bacterial populations of 131 sputa taken from 23 CF patients. These RNAs were blotted and hybridized with probes to P. aeruginosa lasA, lasB, and toxA. The hybridization signals from each probe were ranked, and the rankings were analyzed by a Spearman rank correlation to determine if there was an association between the population transcript accumulations for the three genes. The correlations between the transcript accumulation patterns of pairs of the genes suggested that lasA, lasB, and toxA might be coordinately regulated during CF lung infections. To determine if this coordinate regulation might be due to regulation by LasR, we probed RNAs, extracted from 84 sputa, with the lasR, lasA, lasB, toxA, and algD probes. Statistical analysis indicated that lasR transcript accumulation correlated to lasA, lasB, toxA, and algD transcript accumulations. These results indicated that lasR may at least partially regulate or be coordinately regulated with lasA, lasB, toxA, and algD during the lung infections associated with CF. These results also suggested that the LasR-LasI quorum-sensing system may control the expression of at least some virulence factors in the lungs of patients with CF.

MeSH Terms
ADP Ribose Transferases Adolescent Bacterial Proteins/biosynthesis,genetics Bacterial Toxins Chronic Disease Cystic Fibrosis/complications,microbiology DNA-Binding Proteins/biosynthesis,genetics Exotoxins/biosynthesis,genetics Female Half-Life Humans Lung Diseases/complications,microbiology Male Metalloendopeptidases/biosynthesis,genetics Nucleic Acid Hybridization Pseudomonas Infections/complications,microbiology RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis Respiratory Tract Infections/complications,microbiology Sensitivity and Specificity Trans-Activators/biosynthesis,genetics Transcription, Genetic Virulence Factors
Chemicals
Bacterial Proteins Bacterial Toxins DNA-Binding Proteins Exotoxins LasR protein, Pseudomonas aeruginosa RNA, Bacterial RNA, Messenger Trans-Activators Virulence Factors ADP Ribose Transferases toxA protein, Pseudomonas aeruginosa Metalloendopeptidases pseudolysin, Pseudomonas aeruginosa staphylolytic protease
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Storey D G
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4. storey@acs.ucalgary.ca
Ujack E E
Rabin H R
Mitchell I
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.66.6.2521-2528.1998
Email
storey@acs.ucalgary.ca
Published
1998-06-00
Pages
2521-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC108233
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.66.6.2521-2528.1998 PMC Full Text View journal details
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