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PMID: 8557368 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection.

Infection and immunity ·Vol. 64 ·No. 1 ·1996-01-00 ·Pages 37-43

Tang HB, DiMango E, Bryan R, Gambello M, Iglewski BH, Goldberg JB, Prince A

Abstract

We sought to identify which Pseudomonas aeruginosa products are involved initiating respiratory tract infection. Defined mutants derived from strain PAO i.e., PAOR1 (lasR),PAO-pmm (algC) (an LPS mutant), and AK1152 (which is Fla- and lacks functional pili), were significantly less virulent than PAO1 in a BALBc/ByJ neonatal mouse model of infection as measured by their abilities to cause acute pneumonia, bacteremia, and death. All three mutants were also less adherent to epithelial cells in an in vitro binding assay. PAOR1 and AK1152 were less able to elicit epithelial production of interleukin-8 than PAO1. LasR was found to be required for the optimal expression of neuraminidase under conditions of increased osmolarity, as might be present in certain pathological conditions. PAO-exsA::omega,, which lacks exoenzyme S expression, was fully virulent, causing at least as much pathology as PAO1. The expression of several P. aeruginosa virulence factors appears to be required to establish pulmonary infection in the neonatal mouse.

MeSH Terms
Animals Animals, Newborn Bacterial Adhesion Bacterial Proteins Cells, Cultured DNA-Binding Proteins/genetics Disease Models, Animal Epithelial Cells Fimbriae, Bacterial/genetics Interleukin-8/biosynthesis Lipopolysaccharides/biosynthesis Lung/pathology Mice Mice, Inbred BALB C Mutation Phosphotransferases (Phosphomutases)/genetics Pneumonia, Bacterial/etiology,mortality Pseudomonas Infections/etiology,mortality Pseudomonas aeruginosa/genetics,pathogenicity Trans-Activators/genetics Virulence/genetics
Chemicals
Bacterial Proteins DNA-Binding Proteins Interleukin-8 LasR protein, Pseudomonas aeruginosa Lipopolysaccharides Trans-Activators Phosphotransferases (Phosphomutases) phosphomannomutase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tang H B
Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
DiMango E
Bryan R
Gambello M
Iglewski B H
Goldberg J B
Prince A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.1.37-43.1996
Published
1996-01-00
Pages
37-43
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173724
Subset
IM
Grants
NIDDK NIH HHS · DK36963 · United States
External Links
PubMed source DOI: 10.1128/iai.64.1.37-43.1996 PMC Full Text View journal details
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