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PMID: 9582289 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell cycle modulation of protein-DNA interactions at a human replication origin.

The EMBO journal ·Vol. 17 ·No. 10 ·1998-05-15 ·Pages 2961-9

Abdurashidova G, Riva S, Biamonti G, Giacca M, Falaschi A

Abstract

We followed the variations of protein-DNA interactions occurring in vivo over the early firing replication origin located near the human lamin B2 gene, in IMR-90 cells synchronized in different moments of the cell cycle. In G0 phase cells no protection is present; as the cells progress in G1 phase an extended footprint covering over 100 bp appears, particularly marked at the G1/S border. As the cells enter S phase the protection shrinks to 70 bp and remains unchanged throughout this phase. In mitosis the protection totally disappears, only to reappear in its extended form as the cells move into the next G1. These variations are reminiscent of those corresponding to the formation of the pre- and post-replicative complexes described in yeast and Xenopus cells.

MeSH Terms
Cell Cycle Cells, Cultured DNA/metabolism HeLa Cells Humans Jurkat Cells Lamin Type B Lamins Nuclear Proteins/genetics Promoter Regions, Genetic Proteins/metabolism Replication Origin
Chemicals
Lamin Type B Lamins Nuclear Proteins Proteins lamin B2 DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Abdurashidova G
Molecular Biology Unit, International Centre for Genomic Engineering and Biotechnology, Trieste, Italy.
Riva S
Biamonti G
Giacca M
Falaschi A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.10.2961
Published
1998-05-15
Pages
2961-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170636
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/17.10.2961 PMC Full Text View journal details
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